Electrocatalytic adsorptive voltammetry for fludarabine determination in urine

被引：9

作者：

de-los-Santos-Alvarez, N ^{[1
]}

Lobo-Castañón, MJ ^{[1
]}

Miranda-Ordieres, AJ ^{[1
]}

Tuñón-Blanco, P ^{[1
]}

机构：

[1] Univ Oviedo, Dept Quim Fis & Analit, E-33006 Oviedo, Spain

来源：

ANALYTICA CHIMICA ACTA | 2004年 / 504卷 / 02期

关键词：

fludarabine; NADH; catalytic adsorptive voltammetry;

D O I：

10.1016/j.aca.2003.10.055

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Fludarabine (F-Ara-A, 9-beta-D-arabinofuranosyl-2-fluoroadenine) is an arabinosyl nucleoside with potent cytotoxic activity against chronic lymphocytic leukemia. Herein, its electrochemical behavior is investigated on pyrolytic graphite electrodes (PGEs); and a novel catalytic adsorptive differential-pulse-voltammetric method for its determination is developed based on the strong electrocatalytic effect on nicotinamide adenine dinucleotide (NADH) oxidation exhibited by the oxidation products of F-Ara-A. This approach was applied to human urine samples, which required a previous solid-phase extraction. The F-Ara-A recovery was of 89.7%. The method proposed is fast, simple and permits detection of F-Ara-A concentrations up to 72 h after infusion. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：271 / 277

页数：7

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