Structure of a 129Xe-cryptophane biosensor complexed with human carbonic anhydrase II

被引：46

作者：

Aaron, Julie A. ^{[1
]}

Chambers, Jennifer M. ^{[1
]}

Jude, Kevin M. ^{[1
]}

Di Costanzo, Luigi ^{[1
]}

Dmochowski, Ivan J. ^{[1
]}

Christianson, David W. ^{[1
]}

机构：

[1] Univ Penn, Dept Chem, Roy & Diana Vagelos Labs, Philadelphia, PA 19104 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2008年 / 130卷 / 22期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1021/ja802214x

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Cryptophanes represent an exciting class of xenon-encapsulating molecules that can be exploited as probes for nuclear magnetic resonance imaging. The 1.70 angstrom resolution crystal structure of a cryptophane-derivatized benezenesulfonamide complexed with human carbonic anhydrase 11 shows how an encapsulated xenon atom can be directed to a specific biological target. The crystal structure confirms binding measurements indicating that the cryptophane cage does not strongly interact with the surface of the protein, which may enhance the sensitivity of Xe-129 NMR spectroscopic measurements in solution.

引用

页码：6942 / +

页数：3

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