Photosensitization of singlet oxygen via two-photon-excited fluorescence resonance energy transfer in a water-soluble dendrimer

被引:188
作者
Oar, MA
Serin, JA
Dichtel, WR
Fréchet, JMJ
机构
[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA
[2] SUNY Buffalo, Inst Laser Photon & Biophoton, Buffalo, NY 14260 USA
[3] SUNY Buffalo, Dept Chem, Buffalo, NY 14260 USA
关键词
D O I
10.1021/cm047825i
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
A novel approach for the sensitization of singlet oxygen has been developed which utilizes indirect excitation of the photosensitizer by two-photon-excited fluorescence resonance energy transfer (FRET) from separate chromophores assembled into a dendrimer. This approach effectively enhances the two-photon excitation efficiency of a known photosensitizer, without the sort of chromophore modifications that could lead to loss of photosensitization and other desirable photophysical properties. Photosensitization of singlet oxygen via excitation wavelengths transmissive to human body tissue (750-1000 nm) could alleviate the depth limitations of photodynamic therapy. The dendritic photosensitizer was prepared by grafting two-photon-absorbing chromophores and water-solubilizing moieties to a known multivalent porphyrin photosensitizer. Efficient FRET (> 99% quenching of donor emission) between the peripheral donor two-photon-absorbing chromophores and the central acceptor photosensitizer at the core of the dendrimer was demonstrated under two-photon excitation conditions in an aqueous medium. Photosensitized production of singlet oxygen was monitored through chemical trapping and oxygen luminescence. Both methods independently demonstrated enhanced two-photon-induced singlet oxygen generation upon incorporation of two-photon-absorbing chromophores capable of efficient FRET to the photosensitizer.
引用
收藏
页码:2267 / 2275
页数:9
相关论文
共 20 条
  • [1] Bhawalkar J D, 1997, J Clin Laser Med Surg, V15, P201
  • [3] Photobleaching of sensitisers used in photodynamic therapy
    Bonnett, R
    Martínez, G
    [J]. TETRAHEDRON, 2001, 57 (47) : 9513 - 9547
  • [4] Structure and biodistribution relationships of photodynamic sensitizers
    Boyle, RW
    Dolphin, D
    [J]. PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1996, 64 (03) : 469 - 485
  • [5] 2-PHOTON LASER SCANNING FLUORESCENCE MICROSCOPY
    DENK, W
    STRICKLER, JH
    WEBB, WW
    [J]. SCIENCE, 1990, 248 (4951) : 73 - 76
  • [6] Photosensitized singlet oxygen and its applications
    DeRosa, MC
    Crutchley, RJ
    [J]. COORDINATION CHEMISTRY REVIEWS, 2002, 233 : 351 - 371
  • [7] Singlet oxygen generation via two-photon excited FRET
    Dichtel, WR
    Serin, JM
    Edder, C
    Fréchet, JMJ
    Matuszewski, M
    Tan, LS
    Ohulchanskyy, TY
    Prasad, PN
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (17) : 5380 - 5381
  • [8] DICHTEL WR, UNPUB
  • [9] Drastic enhancement of two-photon absorption in porphyrins associated with symmetrical electron-accepting substitution
    Drobizhev, M
    Karotki, A
    Kruk, M
    Mamardashvili, NZ
    Rebane, A
    [J]. CHEMICAL PHYSICS LETTERS, 2002, 361 (5-6) : 504 - 512
  • [10] Simultaneous two-photon activation of type-I photodynamic therapy agents
    Fisher, WG
    Partridge, WP
    Dees, C
    Wachter, EA
    [J]. PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1997, 66 (02) : 141 - 155