Changes in some pro- and antioxidants in rat cerebellum after chronic alcohol intake

Some pro- and antioxidants were measured in the cerebellum from ethanol-fed rats using ethanol administration in drinking water as a model of moderate alcohol intoxication. After 4 weeks of ethanol intake, a 30% increase in the nonheme iron content in the cerebellum occurred in ethanol-fed rats as compared to control animals. The low molecular-weight chelated iron (LMWC-Fe) content as well as the percentage of total nonheme iron represented by LMWC-Fe were increased in the cerebellar cytosol after chronic ethanol administration. Cerebellar copper and selenium concentrations were lower and zinc concentration higher in ethanol fed rats than in controls. Ethanol consumption decreased the cerebellar vitamin E level. Glutathione S-transferase [EC 2.5.1.18] activity was higher, whereas glutathione peroxidase [glutathione: H2O2 oxidoreductase, EC 1.11.1.9] activity was not altered by ethanol treatment. No significant changes in cerebellar lipid peroxidation, carbonyl protein content, or glutamine synthetase [L-glutamate:ammonia ligase (ADP-forming) EC 6.3.1.2] activity were observed. These results suggest that adaptative increases in some elements of the antioxidant defense may counteract the increase in LMWC-Fe, a pro-oxidant factor, and prevent the occurrence of overt cellular lipid and protein damage. However, after 8 weeks of ethanol intake, the activity of glutamine synthetase, an enzyme specially sensitive to inactivation by oxygen radicals, was decreased, suggesting that this prevention was not totally achieved. (C) 1997 Elsevier Science Inc.