Quantum dot-aptamer nanoprobes for recognizing and labeling influenza A virus particles

被引：62

作者：

Cui, Zong-Qiang ^{[1
]}

Ren, Qian ^{[1
,2
]}

Wei, Hong-Ping ^{[1
]}

Chen, Ze ^{[1
]}

Deng, Jiao-Yu ^{[1
]}

Zhang, Zhi-Ping ^{[1
]}

Zhang, Xian-En ^{[1
]}

机构：

[1] Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

来源：

NANOSCALE | 2011年 / 3卷 / 06期

关键词：

GREEN FLUORESCENT PROTEIN; IN-VITRO SELECTION; HUMAN RHINOVIRUS; GENOMIC RNA; EXPRESSION; INFECTION; BEHAVIOR; LIGANDS; CELLS; DNA;

D O I：

10.1039/c1nr10218d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The fluorescence labeling of viruses is a useful technology for virus detection and imaging. By combining the excellent fluorescence properties of quantum dots (QDs) with the high affinity and specificity of aptamers, we constructed a QD-aptamer probe. The aptamer A22, against the hemagglutinin of influenza A virus, was linked to QDs, producing the QD-A22 probe. Fluorescence imaging and transmission electron microscopy showed that the QD-A22 probe could specifically recognize and label influenza A virus particles. This QD labeling technique provides a new strategy for labeling virus particles for virus detection and imaging.

引用

页码：2454 / 2457

页数：4

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