Chiral capillary electrophoretic separation of selected drugs and metabolites using sulfated β-cyclodextrin

In this paper, we report the chiral separation of some drugs and their metabolites using sulfated beta -cyclodextrin, a powerful chiral selector that has proven to be highly efficient for the separation of chiral drugs, alone or in combination, with other chiral additives. Praziquantel, its metabolite trans-4-hydroxypraziquantel, and albendazole sulfoxide are neutral compounds and could be separated at basic pH (8-10). The simultaneous chiral separation of praziquantel and its metabolite enantiomers was only possible by the addition of sodium deoxycholate to the running buffer. Fluoxetine, disopyramide, and mexiletine, as well as their metabolites are basic compounds and were separated by appropriate selection of the running buffer pH and CD concentration. In addition, the effects of the experimental parameters, such as concentration of sulfated beta -cyclodextrin, pH and buffer concentration, temperature, and voltage were also evaluated. Among the several parameters studied, the concentration of the chiral selector and the pH were the most important to obtain the chiral separation of the selected drugs and metabolites.