High-level expression and purification of recombinant E1 enzyme

被引：8

作者：

Beaudenon, S ^{[1
]}

Huibregtse, JM

机构：

[1] Amb Diagnost, Austin, TX 78744 USA

[2] Univ Texas, Inst Cellular & Mol Biol, Austin, TX 78712 USA

来源：

UBIQUITIN AND PROTEIN DEGRADATION, PART A | 2005年 / 398卷

关键词：

D O I：

10.1016/S0076-6879(05)98001-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The ubiquitin El enzyme is an ATP-dependent enzyme that activates ubiquitin for use in all ubiquitin conjugation pathways. This chapter describes the expression and purification of human E1 enzyme for use in in vitro ubiquitination reactions.

引用

页码：3 / 8

页数：6

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