Simultaneous determination of lamivudine and zidovudine concentrations in human seminal plasma using high-performance liquid chromatography and tandem mass spectrometry

被引：80

作者：

Pereira, AS

Kenney, KB

Cohen, MS

Hall, JE

Eron, JJ

Tidwell, RR

Dunn, JA

机构：

[1] Univ N Carolina, Sch Med, Dept Pathol & Lab Med, Chapel Hill, NC 27599 USA

[2] Glaxo Wellcome Inc, Div Bioanal & Drug Metab, Res Triangle Pk, NC 27709 USA

[3] Univ N Carolina, Sch Med, Div Infect Dis, Chapel Hill, NC 27599 USA

来源：

JOURNAL OF CHROMATOGRAPHY B | 2000年 / 742卷 / 01期

关键词：

lamivudine; zidovudine;

D O I：

10.1016/S0378-4347(00)00162-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A HPLC-MS-MS method was developed and validated to measure lamivudine and zidovudine simultaneously in small volumes of human seminal plasma. Sample preparation was simple and rapid, requiring 25 mu l of sample, the use of isotopically labeled lamivudine and zidovudine as internal standards and ultrafiltration through a molecular mass cut-off membrane. Lamivudine and its internal standard were separated from zidovudine and its internal standard with isocratic HPLC. Detection was carried out using tandem mass spectrometry. This validated method was used to analyze seminal samples obtained from six HIV-positive patients prescribed lamivudine and zidovudine. (C) 2000 Elsevier Science B.V. All rights reserved.

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收藏

页码：173 / 183

页数：11

相关论文

共 24 条

[1] EFFECTS OF DISEASE STAGE AND ZIDOVUDINE THERAPY ON THE DETECTION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 IN SEMEN [J].

ANDERSON, DJ ;

OBRIEN, TR ;

POLITCH, JA ;

MARTINEZ, A ;

SEAGE, GR ;

PADIAN, N ;

HORSBURGH, CR ;

MAYER, KH .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1992, 267 (20) :2769-2774

[2] DETERMINATION OF 3'-AZIDO-3'-DEOXYTHYMIDINE, 2',3'-DIDEOXYCYTIDINE, 3'-FLUORO-3'-DEOXYTHYMIDINE AND 2',3'-DIDEOXYINOSINE IN BIOLOGICAL SAMPLES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

FRIJUSPLESSEN, N ;

MICHAELIS, HC ;

FOTH, H ;

KAHL, GF .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1990, 534 :101-107

[3] Effects of reverse transcriptase inhibitor therapy on the HIV-1 viral burden in semen [J].

Gilliam, BL ;

Dyer, JR ;

Fiscus, SA ;

Marcus, C ;

Zhou, S ;

Wathen, L ;

Freimuth, WW ;

Cohen, MS ;

Eron, JJ .

JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES, 1997, 15 (01) :54-60

[4] FLUORESCENCE POLARIZATION IMMUNOASSAY FOR ZIDOVUDINE [J].

GRANICH, GG ;

EVELAND, MR ;

KROGSTAD, DJ .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1989, 33 (08) :1275-1279

[5] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR 2'-DEOXY-3'-THIACYTIDINE IN HUMAN SERUM [J].

HARKER, AJ ;

EVANS, GL ;

HAWLEY, AE ;

MORRIS, DM .

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1994, 657 (01) :227-232

[6] CONCURRENT ZIDOVUDINE LEVELS IN SEMEN AND SERUM DETERMINED BY RADIOIMMUNOASSAY IN PATIENTS WITH AIDS OR AIDS-RELATED COMPLEX [J].

HENRY, K ;

CHINNOCK, BJ ;

QUINN, RP ;

FLETCHER, CV ;

DEMIRANDA, P ;

BALFOUR, HH .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1988, 259 (20) :3023-3026

[7]

KENNEY KB, IN PRESS J PHARM BIO

[8] RECOVERY OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 FROM SEMEN - MINIMAL IMPACT OF STAGE OF INFECTION AND CURRENT ANTIVIRAL CHEMOTHERAPY [J].

KRIEGER, JN ;

COOMBS, RW ;

COLLIER, AC ;

ROSS, SO ;

CHALOUPKA, K ;

CUMMINGS, DK ;

MURPHY, VL ;

COREY, L .

JOURNAL OF INFECTIOUS DISEASES, 1991, 163 (02) :386-388

[9] HIV-1 detection in cervicovaginal secretions during pregnancy [J].

LoussertAjaka, I ;

Mandelbrot, L ;

Delmas, MC ;

Bastian, H ;

Benifla, JL ;

Farfara, I ;

deVincenzi, I ;

Matheron, S ;

Simon, F ;

BrunVezinet, F .

AIDS, 1997, 11 (13) :1575-1581

[10] DETERMINATION OF 2'-DEOXY-3'-THIACYTIDINE (3TC) IN HUMAN URINE BY LIQUID-CHROMATOGRAPHY - DIRECT-INJECTION WITH COLUMN-SWITCHING [J].

MORRIS, DM ;

SELINGER, K .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1994, 12 (02) :255-264