Microspectroscopy of single proliferating HeLa cells

被引：42

作者：

Boydston-White, S

Chernenko, T

Regina, A

Miljkovic, M

Matthäus, C

Diem, M

机构：

[1] CUNY Hunter Coll, Dept Chem & Biochem, New York, NY 10021 USA

[2] NYU, PhD Program Chem, Grad Sch, New York, NY 10016 USA

[3] NYU, Univ Ctr, New York, NY 10016 USA

来源：

VIBRATIONAL SPECTROSCOPY | 2005年 / 38卷 / 1-2期

关键词：

microspectroscopy; tissue; HeLa cells; cell cycle; FTIR micro-spectroscopy; cell division;

D O I：

10.1016/j.vibspec.2005.02.028

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Spectral differences between normal and abnormal tissue observed to date appear to be due to different averaging processes of spectral patterns that differ according to the cell's biochemistry, due to its state of maturation, differentiation, and development. Thus, disease perturbs the distribution of cells in the different stages of maturation, differentiation, and development. Previous FTIR microspectroscopic studies of normal versus neoplastic cells and tissues have demonstrated differences in the absorption intensities and band-shapes, particularly in the low frequency (1200-1000 cm(-1)) spectral region. In this study, we further investigated the spectral changes due to the drastic biochemical and morphological changes occurring as a consequence of cell proliferation. (c) 2004 Elsevier B.V. All rights reserved.

引用

页码：169 / 177

页数：9

共 21 条

[11] HUMAN CYCLIN-E, A NEW CYCLIN THAT INTERACTS WITH 2 MEMBERS OF THE CDC2 GENE FAMILY [J].