High-molecular-mass complexes of human minichromosome-maintenance proteins in mitotic cells

被引：28

作者：

Richter, A ^{[1
]}

Knippers, R ^{[1
]}

机构：

[1] UNIV KONSTANZ, FAK BIOL, DIV BIOL, D-78457 CONSTANCE, GERMANY

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1997年 / 247卷 / 01期

关键词：

cell division cycle; nuclear protein; minichromosome-maintenance protein; protein phosphorylation; mitosis;

D O I：

10.1111/j.1432-1033.1997.00136.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Minichromosome-maintenance (Mcm) proteins perform essential functions regulating the replication of eukaryotic genomes. In interphase cells they are either bound to a nuclear structure, most probably chromatin, or occur as free multiprotein complexes in the nucleoplasm. Mcm proteins are displaced from their chromatin sites during S phase. and several become highly phosphorylated during mitosis. We investigated whether phosphorylation affects the ability of mitotic Mcm proteins to form multiprotein complexes. Our results clearly show that phosphorylated mitotic Mcm proteins form a 14-15-S complex, probably consisting of one molecule each of the six known human Mcm proteins.

引用

页码：136 / 141

页数：6

共 29 条

[1]

ADAM SA, 1992, METHOD ENZYMOL, V219, P97

[2]

BENSCH KG, 1982, J BIOL CHEM, V257, P8391

[3]

BURKHART R, 1995, EUR J BIOCHEM, V228, P431

[4] PURIFICATION OF AN MCM-CONTAINING COMPLEXES A COMPONENT OF THE DNA-REPLICATION LICENSING SYSTEM [J].

CHONG, JPJ ;

MAHBUBANI, HM ;

KHOO, CY ;

BLOW, JJ .

NATURE, 1995, 375 (6530) :418-421

[5] The Xenopus Cdc6 protein is essential for the initiation of a single round of DNA replication in cell-free extracts [J].

Coleman, TR ;

Carpenter, PB ;

Dunphy, WG .

CELL, 1996, 87 (01) :53-63

[6] Chromatin binding, nuclear localization and phosphorylation of Xenopus cdc21 are cell-cycle dependent and associated with the control of initiation of DNA replication [J].

Coue, M ;

Kearsey, SE ;

Mechali, M .

EMBO JOURNAL, 1996, 15 (05) :1085-1097

[7] Inhibition of S-phase entry of human fibroblasts by an antisense oligomer against hCDC47 [J].

Fujita, M ;

Kiyono, T ;

Hayashi, Y ;

Ishibashi, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 219 (02) :604-607

[8] INTERPHASE CHROMOSOMAL DEOXYRIBONUCLEOPROTEIN ISOLATED AS A DISCRETE STRUCTURE FROM CULTURED-CELLS [J].

HANCOCK, R .

JOURNAL OF MOLECULAR BIOLOGY, 1974, 86 (03) :649-&

[9]

HARLOW E, 1988, ANTIBODIES LAB HDB

[10] A novel human Mcm protein: Homology to the yeast replication protein Mis5 and chromosomal location [J].

Holthoff, HP ;

Hameister, H ;

Knippers, R .

GENOMICS, 1996, 37 (01) :131-134

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