Protein crystal growth with a two-liquid system and stirring solution

被引：42

作者：

Adachi, H

Takano, K

Matsumura, H

Inoue, T

Mori, Y

Sasaki, T

机构：

[1] Osaka Univ, Dept Elect Engn, Suita, Osaka 5650871, Japan

[2] Osaka Univ, SOSHO Project, Crystal Design Project, Suita, Osaka 5650871, Japan

[3] JST, PRESTO, Suita, Osaka 5650871, Japan

[4] Osaka Univ, Dept Mat & Life Sci, Suita, Osaka 5650871, Japan

[5] Osaka Univ, Dept Chem Mat, Suita, Osaka 5650871, Japan

来源：

JOURNAL OF SYNCHROTRON RADIATION | 2004年 / 11卷 / 01期

关键词：

crystal growth; floating; stirring solution; seed technique; large crystal;

D O I：

10.1107/S0909049503026980

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

We developed two novel methods for growing large, high-quality protein crystals. A two-liquid system enables the convenient extraction of protein crystals without causing mechanical damage due to growth at the interface between two liquids. Since this system does not require limitations on solution volume, it is also suitable for the seed technique, and for growing large crystals. Another new concept is the mild stirring of the solution using the Floating And Stirring Technique (FAST) and the Micro-stirring technique. When compared to conventional techniques, both techniques result in a reduced number of crystals, as well as the growth of large crystals.

引用

收藏

页码：121 / 124

页数：4

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