Proteomic profile of culture filtrate from the Brazilian vaccine strain Mycobacterium bovis BCG Moreau compared to M. bovis BCG Pasteur

被引：33

作者：

Berredo-Pinho, Marcia ^{[1
]}

Kalume, Dario E. ^{[2
]}

Correa, Paloma R. ^{[1
]}

Gomes, Leonardo H. F. ^{[1
]}

Pereira, Melissa P. ^{[1
]}

da Silva, Renata F. ^{[1
]}

Castello-Branco, Luiz R. R. ^{[3
]}

Degrave, Wim M. ^{[1
]}

Mendonca-Lima, Leila ^{[1
]}

机构：

[1] Fiocruz MS, Inst Oswaldo Cruz, Lab Genom Func & Bioinformat, BR-21040900 Rio De Janeiro, Brazil

[2] Fiocruz MS, Inst Oswaldo Cruz, Lab Interdisciplinar Pesquisas Med, BR-21040900 Rio De Janeiro, Brazil

[3] Fundacao Ataulpho Paiva FAP, Ctr Pesquisas Arlindo de Assis, Rio De Janeiro, Brazil

来源：

BMC MICROBIOLOGY | 2011年 / 11卷

关键词：

SIGNAL PEPTIDES; TUBERCULOSIS INFECTION; PROTECTIVE IMMUNITY; POLYACRYLAMIDE-GELS; MASS-SPECTROMETRY; PROTEINS; ANTIGEN; IDENTIFICATION; IMMUNIZATION; PREDICTION;

D O I：

10.1186/1471-2180-11-80

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Background: Bacille Calmette-Guerin (BCG) is currently the only available vaccine against tuberculosis (TB) and comprises a heterogeneous family of sub-strains with genotypic and phenotypic differences. The World Health Organization (WHO) affirms that the characterization of BCG sub-strains, both on genomic and proteomic levels, is crucial for a better comprehension of the vaccine. In addition, these studies can contribute in the development of a more efficient vaccine against TB. Here, we combine two-dimensional electrophoresis (2DE) and mass spectrometry to analyse the proteomic profile of culture filtrate proteins (CFPs) from M. bovis BCG Moreau, the Brazilian vaccine strain, comparing it to that of BCG Pasteur. CFPs are considered of great importance given their dominant immunogenicity and role in pathogenesis, being available for interaction with host cells since early infection. Results: The 2DE proteomic map of M. bovis BCG Moreau CFPs in the pH range 3 - 8 allowed the identification of 158 spots corresponding to 101 different proteins, identified by MS/MS. Comparison to BCG Pasteur highlights the great similarity between these BCG strains. However, quantitative analysis shows a higher expression of immunogenic proteins such as Rv1860 (BCG1896, Apa), Rv1926c (BCG1965c, Mpb63) and Rv1886c (BCG1923c, Ag85B) in BCG Moreau when compared to BCG Pasteur, while some heat shock proteins, such as Rv0440 (BCG0479, GroEL2) and Rv0350 (BCG0389, DnaK), show the opposite pattern. Conclusions: Here we report the detailed 2DE profile of CFPs from M. bovis BCG Moreau and its comparison to BCG Pasteur, identifying differences that may provide relevant information on vaccine efficacy. These findings contribute to the detailed characterization of the Brazilian vaccine strain against TB, revealing aspects that may lead to a better understanding of the factors leading to BCG's variable protective efficacy against TB.

引用

页数：12

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