Generation of mouse ES cell lines engineered for the forced induction of transcription factors

被引：39

作者：

Correa-Cerro, Lina S. ^{[1
]}

Piao, Yulan ^{[1
]}

Sharov, Alexei A. ^{[1
]}

Nishiyama, Akira ^{[1
]}

Cadet, Jean S. ^{[1
]}

Yu, Hong ^{[1
]}

Sharova, Lioudmila V. ^{[1
]}

Xin, Li ^{[1
]}

Hoang, Hien G. ^{[1
]}

Thomas, Marshall ^{[1
]}

Qian, Yong ^{[1
]}

Dudekula, Dawood B. ^{[1
]}

Meyers, Emily ^{[1
]}

Binder, Bernard Y. ^{[1
]}

Mowrer, Gregory ^{[1
]}

Bassey, Uwem ^{[1
]}

Longo, Dan L. ^{[1
]}

Schlessinger, David ^{[1
]}

Ko, Minoru S. H. ^{[1
]}

机构：

[1] NIA, NIH, Baltimore, MD 21224 USA

来源：

SCIENTIFIC REPORTS | 2011年 / 1卷

关键词：

GENE SET ENRICHMENT; GENOME-WIDE; EXPRESSION; MICROARRAY; NETWORKS; SYSTEM;

D O I：

10.1038/srep00167

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Here we report the generation and characterization of 84 mouse ES cell lines with doxycycline-controllable transcription factors (TFs) which, together with the previous 53 lines, cover 7-10% of all TFs encoded in the mouse genome. Global gene expression profiles of all 137 lines after the induction of TFs for 48 hrs can associate each TF with the direction of ES cell differentiation, regulatory pathways, and mouse phenotypes. These cell lines and microarray data provide building blocks for a variety of future biomedical research applications as a community resource.

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页数：6

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