Prospective isolation of functionally distinct radial glial subtypes -: Lineage and transcriptome analysis

被引:73
作者
Pinto, Luisa [1 ]
Mader, Michael T. [1 ]
Irmler, Martin [2 ]
Gentilini, Marco [3 ]
Santoni, Federico [4 ]
Drechsel, Daniela [1 ]
Blum, Robert
Stahl, Ronny
Bulfone, Alessandro
Malatesta, Paolo [5 ]
Beckers, Johannes [2 ]
Goetz, Magdalena [1 ,6 ]
机构
[1] German Res Ctr Environm Hlth GmbH, Helmhotz Zentrum Munchen, Inst Stem Cell Res, D-85764 Neuherberg, Germany
[2] German Res Ctr Environm Hlth GmbH, Helmhotz Zentrum Munchen, Inst Expt Genet, D-85764 Neuherberg, Germany
[3] Polaris, Sci & Technol Pk Sardinia, BIOFLAG, Cagliari, Italy
[4] CRS4 Ctr Adv Studies Res & Dev Sardinia, Cagliari, Italy
[5] Ist Nazl Ric Canc, SC Oncol Sperimentale F, I-16132 Genoa, Italy
[6] Univ Munich, Fac Med, D-80633 Munich, Germany
关键词
neurogenesis; gliogenesis; development; cerebral cortex; radial glia; basal precursors; lineage; transcriptome; Pax6; oligodendrogenesis;
D O I
10.1016/j.mcn.2008.01.012
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Since the discovery of radial glia as the source of neurons, their heterogeneity in regard to neurogenesis has been described by clonal and time-lapse analysis in vitro. However, the molecular determinants specifying neurogenic radial glia differently from radial glia that mostly self-renew remain ill-defined. Here, we isolated two radial glial subsets that co-exist at mid-neurogenesis in the developing cerebral cortex and their immediate progeny. While one subset generates neurons directly, the other is largely non-neurogenic but also gives rise to Tbr2-positive basal precursors, thereby contributing indirectly to neurogenesis. Isolation of these distinct radial glia subtypes allowed determining interesting differences in their transcriptome. These transcriptomes were also strikingly different from the transcriptome of radial glia isolated at the end of neurogenesis. This analysis therefore identifies, for the first time, the lineage origin of basal progenitors and the molecular differences of this lineage in comparison to directly neurogenic and gliogenic radial glia. (C) 2008 Elsevier Inc. All rights reserved.
引用
收藏
页码:15 / 42
页数:28
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