Homing of lin-/CD117+ hematopoietic stem cells

被引：8

作者：

Filip, Stanislav ^{[1
]}

Mokry, Jaroslav ^{[2
]}

Vavrova, Jirina ^{[3
]}

Cizkova, Dana ^{[2
]}

Sinkorova, Zuzana ^{[3
]}

Tosnerova, Vlasta ^{[4
]}

Blaha, Milan ^{[5
]}

机构：

[1] Charles Univ Prague, Fac Med & Teaching Hosp, Dept Oncol & Radiotherapy, Hradec Kralove 50005, Czech Republic

[2] Charles Univ Prague, Fac Med, Dept Histol & Embryol, Hradec Kralove 50038, Czech Republic

[3] Univ Def Brno, Dept Radiobiol, Fac Mil Hlth Sci, Hradec Kralove 50005, Czech Republic

[4] Charles Univ Prague, Fac Med & Teaching Hosp, Dept Rehabil Med, Hradec Kralove 50005, Czech Republic

[5] Charles Univ Prague, Fac Med & Teaching Hosp, Dept Hematol, Hradec Kralove 50005, Czech Republic

来源：

TRANSFUSION AND APHERESIS SCIENCE | 2009年 / 41卷 / 03期

关键词：

Hematopoietic stem cells; In vivo homing; Stem cell plasticity; Tissue; Transplantation; Irradiation; BETA-GALACTOSIDASE; MICE; TRANSPLANTATION; RECONSTITUTION; RECOVERY; PROGENITORS; PLASTICITY; INCREASE; RENEWAL; MARKER;

D O I：

10.1016/j.transci.2009.09.011

中图分类号：

R5 [内科学];

学科分类号：

100201 [内科学];

摘要：

In this report, we describe the homing of hematopoietic stem cells (HSCs) to non-hematopoietic tissues in lethally irradiated (9 Gy) hybrid mice transplanted intravenously with lin(-)/CD117(+) bone marrow cells from ROSA26 mice. The numbers of CFU-GM in spleen of irradiated transplanted mice were well above the levels found in non-irradiated group as early as day 8 after transplant. On 12th day regeneration of lymphocytes was observed, an increase in granulocytes was detected as late as on 33rd day. Transplanted cells containing lacZ gene were detected in recipient mice by histochemistry and their location in the thymus, liver, stomach and ileum was followed during 33 days post-transplantation. On 8 and 33 days post-transplantation, we found massive presence of donor (lacZ(+)) cells in the thymic cortex. Hematopoietic stem cell transplantation led not only to recovery of hematopoietic and lymphoid tissues but also facilitated recovery of the small intestinal mucosa, which was significantly damaged by ionizing radiation. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：183 / 190

页数：8

共 29 条

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