Fine-mapping, genomic organization, and transcript analysis of the human ubiquitin-conjugating enzyme gene UBE2L3

被引：18

作者：

Moynihan, TP

Cole, CG

Dunham, L

O'Neil, L

Markham, AF

Robinson, PA

机构：

[1] Univ Leeds, St Jamess Univ Hosp, Mol Med Unit, Leeds LS9 7TF, W Yorkshire, England

[2] Sanger Ctr, Cambridge CB10 1SA, England

来源：

GENOMICS | 1998年 / 51卷 / 01期

基金：

英国惠康基金;

关键词：

D O I：

10.1006/geno.1998.5257

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The human UBE2L3 gene encodes the ubiquitin-conjugating enzyme UbcH7, demonstrated to participate in the ubiquitination of p53, c-Fos, and NF-kappa B in vitro. We report the fine-mapping of this four-exon gene to chromosome 22q11.2. We have constructed a comprehensive genomic clone contig across this gene, demonstrating that the gene lies adjacent to the microsatellite marker D22S446 and spans approximately 57 kb. Four mRNA species are transcribed from this gene, differing in the length of their 3' UTR. Sequence comparison of the UBE2L3 cDNA with its murine homologue reveals a remarkably high degree of sequence conservation within the 3'UTR. (C) 1998 Academic Press.

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页码：124 / 127

页数：4

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