Neuroactive steroids, their precursors, and polar conjugates during parturition and postpartum in maternal and umbilical blood:: I.: identification and simultaneous determination of pregnanolone isomers

被引:37
作者
Hill, M
Parízek, A
Bicíková, M
Havlíková, H
Klak, J
Fait, T
Cibula, D
Hampl, R
Cegan, A
Sulcová, J
Stárka, L
机构
[1] Inst Endocrinol, CZ-11694 Prague, Czech Republic
[2] Charles Univ Prague, Sch Med 1, Clin Cynecol & Obstet, CZ-12851 Prague, Czech Republic
[3] Charles Univ Prague, Gen Hosp, Sch Med 1, CZ-12851 Prague, Czech Republic
[4] Univ Pardubice, Fac Chem Technol, CZ-53210 Pardubice, Czech Republic
关键词
pregnanolone isomers; epipregnanolone; allopregnanolone; polar conjugates; human parturition; GC/MS; maternal serum; umbilical serum;
D O I
10.1016/S0960-0760(00)00192-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A rapid method fur the identification and measurement of four pregnanolone isomers and their polar conjugates in human plasma was developed using a simple quadrupole GC;MS system with electron impact ionization. Steroid levels were measured in the plasma of 13 and three woman at delivery with subarachnoidal and epidural analgesia, respectively, and in corresponding samples of umbilical plasma. A good correlation (r = 0.94. P < 0.001. n = 8) was found between the allopregnanolone in maternal plasma determined by GC MS and that measured by RIA. Epipregnanolone (3<beta>-hydroxy-5 beta -pregenan-20-one) was identified and measured for the first time in human plasma; its concentration in both maternal and umbilical plasma was much lower than that of other pregnanolone isomers. The levels of 3 beta -hydroxy-pregnanolone isomers were significantly higher in the umbilical plasma than in the maternal plasma, while the differences in 3 alpha -hydroxy-isomers were insignificant. The differences in conjugates were insignificant except in the case of allopregnanolone, the levels of which were lower in umbilical plasma. In all of the pregnanolone isomers. a significantly lower conjugated-unconjugated steroid ratio was found in the umbilical plasma than in the maternal plasma. The possible role of the sulfation of pregnanolone isomers around parturition is discussed. (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:237 / 244
页数:8
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