Live Cell Imaging of Mitochondrial Autophagy with a Novel Fluorescent Small Molecule

被引：109

作者：

Iwashita, Hidefumi ^{[1
,2
]}

Torii, Satoru ^{[3
]}

Nagahora, Noriyoshi ^{[2
]}

Ishiyama, Munetaka ^{[1
]}

Shioji, Kosei ^{[2
]}

Sasamoto, Kazumi ^{[1
]}

Shimizu, Shigeomi ^{[3
]}

Okuma, Kentaro ^{[2
]}

机构：

[1] Dojindo Labs, Tabaru 2025-5,Mashiki Machi, Kumamoto 8612202, Japan

[2] Fukuoka Univ, Fac Sci, Dept Chem, Jonan Ku, Fukuoka 8140180, Japan

[3] Tokyo Med & Dent Univ, Med Res Inst, Dept Pathol Cell Biol, Bunkyo Ku, 1-5-45 Yushima, Tokyo 1138510, Japan

来源：

ACS CHEMICAL BIOLOGY | 2017年 / 12卷 / 10期

基金：

日本学术振兴会;

关键词：

MITOPHAGY; PARKIN; PROBE; PH;

D O I：

10.1021/acschembio.7b00647

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

There has been a growing interest in mitophagy, mitochondria-selective autophagy, which plays an essential role in maintaining intracellular homeostasis. We have developed a small-molecule fluorescent probe, Mtphagy Dye, for visualizing mitophagy, which was readily synthesized from a known perylene derivative, perylene-3,4-dicarboxylic anhydride. Mtphagy Dye has suitable fluorescent properties for detecting mitochondrial acidification during mitophagy in the long-wavelength region that does not damage mitochondria. Using Mtphagy Dye, we were able to visualize mitophagy with both cases of Parkin-dependent and -independent HeLa cells.

引用

收藏

页码：2546 / 2551

页数：6

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