Glucose transport by human renal Na+/D-glucose cotransporters SGLT1 and SGLT2

被引:211
作者
Hummel, Charles S. [1 ]
Lu, Chuan [1 ]
Loo, Donald D. F. [1 ]
Hirayama, Bruce A. [1 ]
Voss, Andrew A. [1 ]
Wright, Ernest M. [1 ]
机构
[1] Univ Calif Los Angeles, David Geffen Sch Med, Dept Physiol, Los Angeles, CA 90095 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 2011年 / 300卷 / 01期
基金
美国国家卫生研究院;
关键词
renal glucose reabsorption; Type 2 diabetes mellitus; human Na+/D-glucose cotransporter 2 inhibitors; patch-clamp electrophysiology; NA+/GLUCOSE COTRANSPORTER; HUMAN KIDNEY; LOW-AFFINITY; DIABETES-MELLITUS; GLYCEMIC CONTROL; INHIBITOR; DAPAGLIFLOZIN; TYPE-2; HETEROGENEITY; REABSORPTION;
D O I
10.1152/ajpcell.00388.2010
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Hummel CS, Lu C, Loo DD, Hirayama BA, Voss AA, Wright EM. Glucose transport by human renal Na+/D-glucose cotransporters SGLT1 and SGLT2. Am J Physiol Cell Physiol 300: C14-C21, 2011. First published October 27, 2010; doi: 10.1152/ajpcell. 00388.2010.-The human Na+/D-glucose cotransporter 2 (hSGLT2) is believed to be responsible for the bulk of glucose reabsorption in the kidney proximal convoluted tubule. Since blocking reabsorption increases urinary glucose excretion, hSGLT2 has become a novel drug target for Type 2 diabetes treatment. Glucose transport by hSGLT2 was studied at 37 degrees C in human embryonic kidney 293T cells using whole cell patch-clamp electrophysiology. We compared hSGLT2 with hSGLT1, the transporter in the straight proximal tubule (S3 segment). hSGLT2 transports with surprisingly similar glucose affinity and lower concentrative power than hSGLT1: Na+/D-glucose cotransport by hSGLT2 was electrogenic with apparent glucose and Na+ affinities of 5 and 25 mM, and a Na+ : glucose coupling ratio of 1; hSGLT1 affinities were 2 and 70 mM and coupling ratio of 2. Both proteins showed voltage-dependent steady-state transport; however, unlike hSGLT1, hSGLT2 did not exhibit detectable pre-steady-state currents in response to rapid jumps in membrane voltage. D-Galactose was transported by both proteins, but with very low affinity by hSGLT2 (>= 100 vs. 6 mM). beta-D-Glucopyranosides were either substrates or blockers. Phlorizin exhibited higher affinity with hSGLT2 (K-i 11 vs. 140 nM) and a lower Off-rate (0.03 vs. 0.2 s(-1)) compared with hSGLT1. These studies indicate that, in the early proximal tubule, hSGLT2 works at 50% capacity and becomes saturated only when glucose is >= 35 mM. Furthermore, results on hSGLT1 suggest it may play a significant role in the reabsorption of filtered glucose in the late proximal tubule. Our electrophysiological study provides groundwork for a molecular understanding of how hSGLT inhibitors affect renal glucose reabsorption.
引用
收藏
页码:C14 / C21
页数:8
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