Effects of brazilin on the phospholipase A2 activity and changes of intracellular free calcium concentration in rat platelets

被引：44

作者：

Hwang, GS

Kim, JY

Chang, TS

Jeon, SD

So, DS

Moon, CK ^{[1
]}

机构：

[1] Seoul Natl Univ, Coll Pharm, Kwanak Gu, Seoul 151742, South Korea

[2] Kyung Won Univ, Coll Oriental Med, Seongnam, South Korea

来源：

ARCHIVES OF PHARMACAL RESEARCH | 1998年 / 21卷 / 06期

关键词：

brazilin; Caesalpinia sappan; aggregation; phospholipase A(2); platelet; intracellular; free calcium;

D O I：

10.1007/BF02976775

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Brazilin {7,11b-dihydrobenz[b]indeno[1,2-d]pyran-3,6a,9,10(6H)-tetrol} inhibited thrombin-, collagen- and ADP-induced aggregation of washed rat platelets. Thrombin- and collagen-induced ATP release were also inhibited by brazilin in a concentration-dependent manner. Brazilin inhibited the formation of platelet thromboxane A(2) caused by thrombin, whereas it had no effect on the prostaglandin D-2 formation. Brazilin inhibited [H-3]-arachidonic acid liberation from membrane phospholipids of thrombin-stimulated platelets. Brazilin inhibited the rise of intracellular free calcium caused by thrombin. These results indicate that the inhibition of phospholipase (PLA(2)) activity and [Ca2+](i) elevation might be at least a part of antiplatelet mechanism of brazilin.

引用

页码：774 / 778

页数：5