Efficient generation of transgenic BALB/c mice using BALB/c embryonic stem cells

被引：24

作者：

Dinkel, A

Aicher, WK

Warnatz, K

Bürki, K

Eibel, H

Ledermann, B ^{[1
]}

机构：

[1] Novartis Pharma, Preclin Res, CH-4002 Basel, Switzerland

[2] Univ Freiburg, Med Ctr, Clin Res Unit Rheumatol, D-79106 Freiburg, Germany

[3] Univ Freiburg, Dept Biol, D-79108 Freiburg, Germany

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1999年 / 223卷 / 02期

关键词：

BALB/c; transgenic; embryonic stem cells; Egr-1; B cells;

D O I：

10.1016/S0022-1759(99)00005-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

BALB/c is one of the most widely used and best characterized mouse strains in immunology. For various applications, it is necessary to generate BALB/c transgenic mice. However, using the conventional microinjection technique it is extremely inefficient to produce transgenic BALB/c mice since the one-cell stage BALB/c embryos are highly vulnerable to pronuclear DNA microinjection. To overcome this problem, we have investigated the generation of Egr-1 (early growth response gene) transgenic mice via the transfection of BALB/c embryonic stem cells. Transfectants carrying Egr-1 constructs comprising either the immunoglobulin heavy chain or the MHC class II promoter/enhancer system were injected into C57BL/6 host blastocysts resulting in chimeric mice. For both type of expression vectors, transgenic offspring of the germline chimeras expressed recombinant Egr-1 in lymphoid tissues containing B cells. This demonstrates the successful generation of Egr-1 transgenic BALB/c mice using transfected ES cell. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：255 / 260

页数：6

共 14 条

[11] DIFFERENTIAL EXPRESSION OF A ZINC FINGER-ENCODING GENE IN RESPONSE TO POSITIVE VERSUS NEGATIVE SIGNALING THROUGH RECEPTOR IMMUNOGLOBULIN IN MURINE LYMPHOCYTES-B [J].