Human recombinant single-chain antibody fragments, specific for the hypervariable region 1 of hepatitis C virus, from immune phage-display libraries

被引:11
作者
Zhai, W
Davies, J
Shang, DZ
Chan, SW
Allain, JP
机构
[1] Univ Cambridge, Dept Haematol, Div Transfus Med, Cambridge CB2 1TN, England
[2] Univ Cambridge, Addenbrookes Hosp, Dept Med, Cambridge CB2 2QQ, England
关键词
antibody; HCV; HVR1; peptides; scFv;
D O I
10.1046/j.1365-2893.1999.00146.x
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
The hypervariable region 1 (HVR1) of hepatitis C virus (HCV) may contain a potential neutralization site and the generation of human single-chain antibody fragments (scFv) to HVR1 may therefore provide a useful tool for the study of HCV. In this report, we have isolated and characterized three anti-HVR1 scFv clones from two patient-derived phage-displayed libraries and HCV HVR1 peptides. scFv S52/20 and S53/6 were selected with serologically cross-reactive HVR1 peptides. scFv p3f10 was obtained by screening the library from patient MH with an autologous HVR1 peptide. Nucleotide sequencing showed that the V-H chains and V kappa chains of all three scFv antibodies were derived from V(H)3 and V kappa 1 family germline V-genes, respectively. The specificity and affinity of the recombinant scFv antibodies were examined by enzyme-linked immunosorbent assay (ELISA) and an affinity biosensor, using HVR1 peptides, S52/20 scFv binding to S52 HVR1 peptide was blocked by preincubation with soluble peptide S52 and was partially competed by one of three HCV-infected patient sera. In addition, scFv S52/20 blocked the binding of HCV-susceptible Molt-4 cells to immobilized S52 peptide. This study demonstrates that recombinant human scFv antibodies to HCV HVR1 can be produced in vito and directly confirms that HVR1 of HCV elicits highly specific antibodies. The very high specificity of these antibodies to HVR1 may limit their potential use in passive immunization therapy against HCV, and further engineering of the scFvs needs to be performed to generate broad-spectrum blocking scFvs.
引用
收藏
页码:115 / 124
页数:10
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