Functional interplay between Parp-1 and SirT1 in genome integrity and chromatin-based processes

被引:45
作者
El Ramy, Rosy [1 ]
Magroun, Najat [1 ]
Messadecq, Nadia [2 ]
Gauthier, Laurent R. [3 ]
Boussin, Francois D. [3 ]
Kolthur-Seetharam, Ullas [4 ]
Schreiber, Valerie [1 ]
McBurney, Michael W. [5 ]
Sassone-Corsi, Paolo [6 ]
Dantzer, Francoise [1 ]
机构
[1] ESBS, IREBS FRE3211, F-67412 Illkirch Graffenstaden, France
[2] IGBMC, F-67404 Illkirch Graffenstaden, France
[3] INSERM, Lab Radiopathol, CEA, IRCM,U967, F-92265 Fontenay Aux Roses, France
[4] Tata Inst Fundamental Res, Dept Biol Sci, Bombay 400005, Maharashtra, India
[5] Univ Ottawa, Ctr Canc Therapeut, Ottawa Hlth Res Inst, Ottawa, ON, Canada
[6] Univ Calif Irvine, Dept Pharmacol, GNRF, Irvine, CA 92697 USA
关键词
Poly(ADP-ribosyl)ation; Acetylation; Genome integrity; Chromatin modifications; Sirtuins; POLY(ADP-RIBOSE) POLYMERASE-1; HISTONE DEACETYLASE; DNA-DAMAGE; TELOMERE LENGTH; CELL-DEATH; PROTEIN; HETEROCHROMATIN; MICE; STABILITY; ACETYLATION;
D O I
10.1007/s00018-009-0105-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Poly(ADP-ribose) polymerase-1 (Parp-1) and the protein deacetylase SirT1 are two of the most effective NAD(+)-consuming enzymes in the cell with key functions in genome integrity and chromatin-based pathways. Here, we examined the in vivo crosstalk between both proteins. We observed that the double disruption of both genes in mice tends to increase late post-natal lethality before weaning consistent with important roles of both proteins in genome integrity during mouse development. We identified increased spontaneous telomeric abnormalities associated with decreased cell growth in the absence of either SirT1 or SirT1 and Parp-1 in mouse cells. In contrast, the additional disruption of Parp-1 rescued the abnormal pericentric heterochromatin, the nucleolar disorganization and the mitotic defects observed in SirT1-deficient cells. Together, these findings are in favor of key functions of both proteins in cellular response to DNA damage and in the modulation of histone modifications associated with constitutive heterochromatin integrity.
引用
收藏
页码:3219 / 3234
页数:16
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