Identification of drought-induced microRNAs in rice

被引：433

作者：

Zhao, Botao

Liang, Ruqiang

Ge, Liangfa

Li, Wei

Xiao, Huasheng

Lin, Hongxuan ^{[1
]}

Ruan, Kangcheng

Jin, Youxin

机构：

[1] Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai 200031, Peoples R China

[2] Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, Shanghai 200032, Peoples R China

[3] Chinese Acad Sci, Inst Biol Sci, Shanghai 200031, Peoples R China

[4] Natl Engn Ctr Biochip Shanghai, Zhangjiang High Tech Pk, Shanghai 201203, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 354卷 / 02期

基金：

中国国家自然科学基金;

关键词：

rice; drought stress; microRNA; miR-169; DRE; CBF/DREB;

D O I：

10.1016/j.bbrc.2007.01.022

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

MicroRNAs (miRNAs) are a large new class of small non-coding RNAs. To date, hundreds of microRNAs have been identified in plants. NlicroRNAs play important roles in post-transcriptional gene regulation by targeting mRNAs for cleavage or repressing translation. To better understand microRNA function, we have used an oligonucleotide microarray to monitor rice (Oryza sativa) microRNA expression profile under drought stress. Two drought-induced microRNAs were identified. Furthermore, miR-169g was confirmed as the only member induced by drought among the miR-169 family and the induction of miR-169g was more prominent in roots than in shoots. Sequence analysis revealed occurrence of two proximate DREs (dehydration-responsive element) in the upstream of the MIR-169g, suggesting that miR-169g expression may be regulated directly by CBF/DREBs. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：585 / 590

页数：6

共 26 条

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