Characterization of tBid-induced cytochrome c release from mitochondria and liposomes

被引：39

作者：

Zhai, DY ^{[1
]}

Huang, XX ^{[1
]}

Han, XH ^{[1
]}

Yang, FY ^{[1
]}

机构：

[1] Acad Sinica, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China

来源：

FEBS LETTERS | 2000年 / 472卷 / 2-3期

基金：

中国国家自然科学基金;

关键词：

apoptosis; bid; cytochrome c release; mitochondrion; permeability transition pore; liposome;

D O I：

10.1016/S0014-5793(00)01471-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

tBid, the cleaved form of Bid, can induce cytochrome c (Cyt, c) release from rat heart mitochondria more efficiently and reproducibly than that from liver or brain mitochondria, Unlike Bas, such release was not prevented by cyclosphorin A, an inhibitor of the opening of permeability transition pore. Carbonyl-cyanide m-chlorophenyl-hydrazone or oligomycin also have no obvious effect on the release of Cyt. c, In contrast to ceramide, tBid-mediated Cyt. c release from mitochondria is independent of the redox state of Cyt. c. Furthermore, Bid or tBid can directly trigger the efflux of encapsulated Cyt, c or trypsin within liposomes without involvement of other protein factors. (C) 2000 Federation of European Biochemical Societies.

引用

页码：293 / 296

页数：4

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