Differences in expression of toll-like receptors and their reactivities in dendritic cells in BALB/c and C57BL/6 mice

被引:126
作者
Liu, T
Matsuguchi, T
Tsuboi, N
Yajima, T
Yoshikai, Y [1 ]
机构
[1] Kyushu Univ, Div Host Def, Res Ctr Prevent Infect Dis, Med Inst Bioregulat, Fukuoka 8128585, Japan
[2] Nagoya Univ, Host Def Lab, Dis Mechanism & Control Res Inst, Grad Sch Med, Nagoya, Aichi 4668550, Japan
关键词
D O I
10.1128/IAI.70.12.6638-6645.2002
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We have previously reported that differences in early production of interleukin 12 (IL-12) by dendritic cells (DC) underlies the difference between the susceptibilities to Listeria monocytogenes of C57BL/6 and BALB/c mice. To elucidate mechanisms for the different abilities of DC to produce cytokine in C57BL/6 and BALB/c mice, we examined Toll-like receptor (TLR) expression by DC and their responses in vitro to known microbial ligands for TLRs. We found that DC isolated from the spleens of naive C57BL/6 mice preferentially expressed TLR9 mRNA, whereas DC from naive BALB/c mice strongly expressed TLR2, -4, -5, and -6 mRNAs. C57BL/6 DC produced a higher level of IL-12p40 in response to the ligands for TLR4 (lipopolysaccharide), TLR2 (lipoprotein), and TLR9 (CpG), whereas BALB/c DC responded to these ligands by producing a larger amount of monocyte chemoattractant protein 1. C57BL/6 DC expressed higher levels of CD40 and Stat4 than BALB/c DC did, suggesting that naive C57BL/6 mice contained more-mature subsets of DC than naive BALB/c mice. Differences in reactivities of DC to microbial molecules through TLRs may be associated with susceptibility and resistance to Listeria infection in BALB/c and C57BL/6 mice.
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页码:6638 / 6645
页数:8
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