LIPS arrays for simultaneous detection of antibodies against partial and whole proteomes of HCV, HIV and EBV

被引：26

作者：

Burbelo, Peter D. ^{[1
]}

Bren, Kathleen E. ^{[1
]}

Ching, Kathryn H. ^{[1
]}

Gogineni, Emile S. ^{[2
]}

Kottilil, Shyam ^{[3
]}

Cohen, Jeffrey I. ^{[4
]}

Kovacs, Joseph A. ^{[2
]}

Iadarola, Michael J. ^{[1
]}

机构：

[1] Natl Inst Dent & Craniofacial Res, Neurobiol & Pain Therapeut Sect, Lab Sensory Biol, NIH, Bethesda, MD 20892 USA

[2] NIH, Dept Crit Care Med, Ctr Clin, Bethesda, MD 20892 USA

[3] NIAID, Immunoregulat Lab, NIH, Bethesda, MD 20892 USA

[4] NIAID, Med Virol Sect, Infect Dis Lab, NIH, Bethesda, MD 20892 USA

来源：

MOLECULAR BIOSYSTEMS | 2011年 / 7卷 / 05期

关键词：

HUMORAL IMMUNE-RESPONSE; PROTEIN MICROARRAYS; INFECTIOUS AGENTS; PROFILES; DISEASE; VACCINE;

D O I：

10.1039/c0mb00342e

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

For many infectious agents, the detection of antibodies is critical for diagnosing, monitoring and understanding vaccine responses. To facilitate the highly quantitative and simultaneous analysis of antibodies against multiple proteins from infectious agents, we have developed Luciferase Immunoprecipitation Systems (LIPS) arrays. By configuring microtiter plates with multiple antigens and testing control and infected serum samples at one time in solution, LIPS arrays provided highly reproducible antibody titers to panels of antigens with a wide dynamic range of detection. While all serum samples showed similar positive and negative immunoreactivity with internal control antigens derived from Influenza and Renilla luciferase-alone protein, respectively, antibody titers to many HCV and HIV antigens were generally 10 to over 400-fold higher in the infected versus uninfected samples. Additional screening of 18 proteins from the EBV proteome with serum samples from healthy EBV-infected individuals showed statistically significant antibody titers to 50% of the proteins tested. Antibody titers for the different EBV antigens in the healthy EBV-infected individuals were markedly heterogeneous highlighting the complexity of host humoral responses. These results suggest that LIPS arrays offer a highly discriminating platform for simultaneously profiling a wide spectrum of antibodies associated with many infectious agents.

引用

页码：1453 / 1462

页数：10

共 25 条

[11] A Four-Antigen Mixture for Rapid Assessment of Onchocerca volvulus Infection [J].

Burbelo, Peter D. ;

Leahy, Hannah P. ;

Iadarola, Michael J. ;

Nutman, Thomas B. .

PLOS NEGLECTED TROPICAL DISEASES, 2009, 3 (05) :e438

[12] Four-Antigen Mixture Containing V-Cyclin for Serological Screening of Human Herpesvirus 8 Infection [J].

Burbelo, Peter D. ;

Leahy, Hannah P. ;

Groot, Sandra ;

Bishop, Lisa R. ;

Miley, Wendell ;

Iadarola, Michael J. ;

Whitby, Denise ;

Kovacs, Joseph A. .

CLINICAL AND VACCINE IMMUNOLOGY, 2009, 16 (05) :621-627

[13] Anti-HTLV antibody profiling reveals an antibody signature for HTLV-I-Associated Myelopathy/Tropical Spastic Paraparesis (HAM/TSP) [J].

Burbelo, Peter D. ;

Meoli, Elise ;

Leahy, Hannah P. ;

Graham, Jhanelle ;

Yao, Karen ;

Oh, Unsong ;

Janik, John E. ;

Mahieux, Renaud ;

Kashanchi, Fatah ;

Iadarola, Michael J. ;

Jacobson, Steven .

RETROVIROLOGY, 2008, 5 (1)

[14] Systematic analysis of the IgG antibody immune response against varicella zoster virus (VZV) using a self-assembled protein microarray [J].

Ceroni, Alessandro ;

Sibani, Sahar ;

Baiker, Armin ;

Pothineni, Venkata Raveendra ;

Bailer, Susanne M. ;

LaBaer, Joshua ;

Haas, Juergen ;

Campbell, Colin J. .

MOLECULAR BIOSYSTEMS, 2010, 6 (09) :1604-1610

[15] Profiling the humoral immune response to infection by using proteome microarrays: High-throughput vaccine and diagnostic antigen discovery [J].

Davies, DH ;

Liang, XW ;

Hernandez, JE ;

Randall, A ;

Hirst, S ;

Mu, YX ;

Romero, KM ;

Nguyen, TT ;

Kalantari-Dehaghi, M ;

Crotty, S ;

Baldi, P ;

Villarreal, LP ;

Felgner, PL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (03) :547-552

[16] Profiling humoral immune responses to P-falciparum infection with protein microarrays [J].

Doolan, Denise L. ;

Mu, Yunxiang ;

Unal, Berkay ;

Sundaresh, Suman ;

Hirst, Siddiqua ;

Valdez, Conrad ;

Randall, Arlo ;

Molina, Douglas ;

Liang, Xiaowu ;

Freilich, Daniel A. ;

Oloo, J. Aggrey ;

Blair, Peter L. ;

Aguiar, Joao C. ;

Baldi, Pierre ;

Davies, D. Huw ;

Felgner, Philip L. .

PROTEOMICS, 2008, 8 (22) :4680-4694

[17] Proteome-wide analysis of the serological response to vaccinia and smallpox [J].

Huw Davies, D. ;

Molina, Douglas M. ;

Wrammert, Jens ;

Miller, Joe ;

Hirst, Siddiqua ;

Mu, Yunxiang ;

Pablo, Jozelyn ;

Unal, Berkay ;

Nakajima-Sasaki, Rie ;

Liang, Xiaowu ;

Crotty, Shane ;

Karem, Kevin L. ;

Damon, Inger K. ;

Ahmed, Rafi ;

Villarreal, Luis ;

Felgner, Philip L. .

PROTEOMICS, 2007, 7 (10) :1678-1686

[18] Spectrum of Epstein-Barr virus-associated diseases [J].

Kutok, J. L. ;

Wang, F. .

ANNUAL REVIEW OF PATHOLOGY-MECHANISMS OF DISEASE, 2006, 1 (01) :375-404

[19] Accepting clocks that tell time poorly: Fluid-phase versus standard ELISA autoantibody assays [J].

Liu, Edwin ;

Eisenbarth, George S. .

CLINICAL IMMUNOLOGY, 2007, 125 (02) :120-126

[20]

Matsuyama A, 2009, METHODS MOL BIOL, V577, P11, DOI 10.1007/978-1-60761-232-2_2

← 1 2 3 →