Effect of annealing temperature on antimicrobial and structural properties of bio-synthesized zinc oxide nanoparticles using flower extract of Anchusa italica

被引：115

作者：

Azizi, Susan ^{[1
]}

Mohamad, Rosfarizan ^{[1
,2
]}

Bahadoran, Azadeh ^{[3
,4
]}

Bayat, Saadi ^{[5
,6
]}

Rahim, Raha Abdul ^{[7
]}

Ariff, Arbakariya ^{[1
]}

Saad, Wan Zuhainis ^{[8
]}

机构：

[1] Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Bioproc Technol, Upm Serdang 43400, Selangor, Malaysia

[2] Univ Putra Malaysia, Inst Trop Forestry & Forest Prod INTROP, Upm Serdang 43400, Selangor, Malaysia

[3] Univ Putra Malaysia, Inst Biosci, Upm Serdang 43400, Selangor, Malaysia

[4] Univ Malaya, Fac Med, Dept Med Microbiol, Kuala Lumpur, Malaysia

[5] La Trobe Inst Mol Sci, Dept Chem & Phys, Melbourne, Vic, Australia

[6] Univ Putra Malaysia, Dept Chem, Upm Serdang 43400, Selangor, Malaysia

[7] Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Cell & Mol Biol, Upm Serdang 43400, Selangor, Malaysia

[8] Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Microbiol, Upm Serdang 43400, Selangor, Malaysia

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 2016年 / 161卷

关键词：

Green synthesis; ZnO nanoparticles; Anchusa italica; Antimicrobial; annealing temperature; ZNO NANOPARTICLES; ANTIBACTERIAL ACTIVITY; GREEN SYNTHESIS; ESCHERICHIA-COLI; ESSENTIAL OIL; LEAF EXTRACT; BIOSYNTHESIS; BACTERIA; SUSCEPTIBILITY; ANTIOXIDANT;

D O I：

10.1016/j.jphotobiol.2016.06.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The use of nontoxic biological compounds in the synthesis of nanomaterials is an economic and eco-friendly approach. The present work was undertaken to develop zinc oxide nanoparticles (ZnO-NPs) by a green method using simple precursor from the solution consisting of zinc acetate and the flower extract of Anchusa italica (A. italica). Effect of annealing temperature on structural and antimicrobial properties was investigated. The crystalline structure of ZnO-NPs was shown using X-ray diffraction (XRD) analysis. Transmission electron microscopy (TEM) results showed that ZnO-NPs are hexagonal in shapes with mean particle size of similar to 8 and similar to 14 nm at 100 degrees C and 200 degrees C annealing temperatures respectively. The optical band gap was increased from 3.27 eV to 3.30 eV with the decreasing of the particle size. The antimicrobial activity of ZnO-NPs towards Gram positive (Bacillus megaterium and Stapphylococcus aureus) and Gram negative (Escherichia coli and Salmonella typhimurium) pathogens decreased with the increasing of the heat treating temperature. In vitro cytotoxicity studies on Vero cells, a dose dependent toxicity with non-toxic effect of concentration below 142 mu g/mL was shown. The results indicated that A. italica is an appropriate reaction media to prepare ZnO-NPs for cosmetic and bio-medical productions. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：441 / 449

页数：9

共 49 条

[1]

Abbas M, 2009, PHARM SCI, V2, P131

[2]

Antioxidant activity and total phenolic content of aqueous and methanolic extracts of Jordanian plants: an ICBG project [J].