Microbatch macromolecular crystallization in micropipettes

被引：14

作者：

Luft, JR ^{[1
]}

Rak, DM ^{[1
]}

DeTitta, GT ^{[1
]}

机构：

[1] Hauptman Woodward Med Res Inc, Buffalo, NY 14203 USA

来源：

JOURNAL OF CRYSTAL GROWTH | 1999年 / 196卷 / 2-4期

基金：

美国国家航空航天局; 美国国家卫生研究院;

关键词：

crystallization; macromolecule; microbatch; micropipette;

D O I：

10.1016/S0022-0248(98)00864-1

中图分类号：

O7 [晶体学];

学科分类号：

0702 ; 070205 ; 0703 ; 080501 ;

摘要：

A microbatch crystallization method suitable for macromolecular crystal growth is described. Solutions in the 1 mu l range are set up in micropipettes with very small inner diameter. The packaging is robust, compact and neat. Surface area of solution exposed to the atmosphere is much smaller than in a hanging drop vapor diffusion experiment. Small sample volumes make the packaging ideal for screening experiments, but crystals large enough for diffraction studies can be grown in? and recovered from, the micropipettes. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：450 / 455

页数：6

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