Immobilisation of thiabendazole specific antibodies on an agarose matrix for application in immunoaffinity chromatography

被引:9
作者
Horne, E
Coyle, T
O'Keeffe, M [1 ]
Brandon, DL
机构
[1] TEAGASC, Natl Food Ctr, Dublin 15, Ireland
[2] ARS, Food Safety & Hlth Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA
关键词
D O I
10.1039/a805220d
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The covalent immobilisation of two monoclonal antibodies teach of which binds both thiabendazole and its metabolite, 5-hydroxythiabendazole) on an agarose matrix is described. Immunoaffinity columns, made using these immunosorbents, bound radiolabelled thiabendazole and a number of eluents, were evaluated, with ethanol-water (70%) being selected as the most suitable. Re-equilibration of the immunosorbent was possible by purging with water, resulting in a reusable column. The columns were found to be stable under the conditions employed.
引用
收藏
页码:87 / 90
页数:4
相关论文
共 13 条
[1]   MONOCLONAL ANTIBODY-BASED ELISA FOR THIABENDAZOLE IN LIVER [J].
BRANDON, DL ;
BINDER, RG ;
BATES, AH ;
MONTAGUE, WC .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1992, 40 (09) :1722-1726
[2]  
Courtheyn D., 1993, P EURORESIDUE 2 C 3, VII, P251
[3]   ONLINE IMMUNOAFFINITY SAMPLE PRETREATMENT FOR COLUMN LIQUID-CHROMATOGRAPHY - EVALUATION OF DESORPTION TECHNIQUES AND OPERATING-CONDITIONS USING AN ANTIESTROGEN IMMUNO-PRECOLUMN AS A MODEL SYSTEM [J].
FARJAM, A ;
BRUGMAN, AE ;
LINGEMAN, H ;
THBRINKMAN, UA .
ANALYST, 1991, 116 (09) :891-896
[4]   DETERMINATION OF BETA-19-NORTESTOSTERONE AND ITS METABOLITE ALPHA-19-NORTESTOSTERONE IN BIOLOGICAL SAMPLES AT THE SUB PARTS PER BILLION LEVEL BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ONLINE IMMUNOAFFINITY SAMPLE PRETREATMENT [J].
HAASNOOT, W ;
SCHILT, R ;
HAMERS, ARM ;
HUF, FA ;
FARJAM, A ;
FREI, RW ;
BRINKMAN, UAT .
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1989, 489 (01) :157-171
[5]  
Li JS, 1996, J AOAC INT, V79, P1062
[6]  
*PIERC WARR, TECHN GUID PROT IMM
[7]   COMBINED IMMUNOEXTRACTION APPROACH COUPLED TO A CHEMILUMINESCENCE ENZYME-IMMUNOASSAY FOR THE DETERMINATION OF TRACE LEVELS OF SALBUTAMOL AND CLENBUTEROL IN TISSUE SAMPLES [J].
POU, K ;
ONG, H ;
ADAM, A ;
LAMOTHE, P ;
DELAHAUT, P .
ANALYST, 1994, 119 (12) :2659-2662
[8]   COMPARISON OF IMMUNOAFFINITY CHROMATOGRAPHY COMBINED WITH GAS-CHROMATOGRAPHY NEGATIVE-ION CHEMICAL-IONIZATION MASS-SPECTROMETRY AND RADIOIMMUNOASSAY FOR SCREENING DEXAMETHASONE IN EQUINE URINE [J].
STANLEY, SMR ;
WILHELMI, BS ;
RODGERS, JP .
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1993, 620 (02) :250-253
[9]   DETERMINATION OF CHLORAMPHENICOL IN SWINE MUSCLE-TISSUE USING A MONOCLONAL ANTIBODY-MEDIATED CLEANUP PROCEDURE [J].
VANDEWATER, C ;
HAAGSMA, N .
JOURNAL OF CHROMATOGRAPHY, 1987, 411 :415-421
[10]   MONOCLONAL ANTIBODY-MEDIATED CLEANUP PROCEDURE FOR THE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF CHLORAMPHENICOL IN MILK AND EGGS [J].
VANDEWATER, C ;
TEBBAL, D ;
HAAGSMA, N .
JOURNAL OF CHROMATOGRAPHY, 1989, 478 (01) :205-215