Using conditional mutagenesis to study the brain

被引：54

作者：

Morozov, A

Kellendonk, C

Simpson, E

Tronche, F

机构：

[1] NIMH, Unit Behav Genet, Mol Pathophysiol Lab, US Dept HHS,NIH, Bethesda, MD 20892 USA

[2] Columbia Univ, Ctr Neurobiol & Behav, New York, NY 10032 USA

[3] Columbia Univ, Howard Hughes Med Inst, New York, NY 10032 USA

[4] Coll France, Inst Biol, CNRS, F-75231 Paris, France

来源：

BIOLOGICAL PSYCHIATRY | 2003年 / 54卷 / 11期

关键词：

gene targeting; Cre recombinase; tetracycline; transactivator; brain;

D O I：

10.1016/S0006-3223(03)00467-0

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Conditional genetic modifications are used to determine how individual molecules contribute to the function of defined neuronal circuits in the mouse brain. Among various techniques for these genetic modifications, the tetracycline transactivator and the Cre-loxP systems have proved to be most successful in recent years. Here we describe the basic principles, recent developments, and potential applications of these methodologies. We discuss their impact on the study of general brain function and their use for modeling different brain disorders.

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页码：1125 / 1133

页数：9

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