Using conditional mutagenesis to study the brain

被引：54

作者：

Morozov, A

Kellendonk, C

Simpson, E

Tronche, F

机构：

[1] NIMH, Unit Behav Genet, Mol Pathophysiol Lab, US Dept HHS,NIH, Bethesda, MD 20892 USA

[2] Columbia Univ, Ctr Neurobiol & Behav, New York, NY 10032 USA

[3] Columbia Univ, Howard Hughes Med Inst, New York, NY 10032 USA

[4] Coll France, Inst Biol, CNRS, F-75231 Paris, France

来源：

BIOLOGICAL PSYCHIATRY | 2003年 / 54卷 / 11期

关键词：

gene targeting; Cre recombinase; tetracycline; transactivator; brain;

D O I：

10.1016/S0006-3223(03)00467-0

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Conditional genetic modifications are used to determine how individual molecules contribute to the function of defined neuronal circuits in the mouse brain. Among various techniques for these genetic modifications, the tetracycline transactivator and the Cre-loxP systems have proved to be most successful in recent years. Here we describe the basic principles, recent developments, and potential applications of these methodologies. We discuss their impact on the study of general brain function and their use for modeling different brain disorders.

引用

收藏

页码：1125 / 1133

页数：9

相关论文

共 70 条

[21] DELETION OF A DNA-POLYMERASE-BETA GENE SEGMENT IN T-CELLS USING CELL-TYPE-SPECIFIC GENE TARGETING [J].

GU, H ;

MARTH, JD ;

ORBAN, PC ;

MOSSMANN, H ;

RAJEWSKY, K .

SCIENCE, 1994, 265 (5168) :103-106

[22] Long-term, noninvasive imaging of regulated gene expression in living mice [J].

Hasan, MT ;

Schönig, K ;

Berger, S ;

Graewe, W ;

Bujard, H .

GENESIS, 2001, 29 (03) :116-122

[23] Targeting of cre to the Foxg1 (BF-1) locus mediates loxP recombination in the telencephalon and other developing head structures [J].

Hébert, JM ;

McConnell, SK .

DEVELOPMENTAL BIOLOGY, 2000, 222 (02) :296-306

[24] Neuron-specific expression of Cre recombinase during the late phase of brain development [J].

Hirasawa, M ;

Cho, A ;

Sreenath, T ;

Sauer, B ;

Julien, JP ;

Kulkarni, AB .

NEUROSCIENCE RESEARCH, 2001, 40 (02) :125-132

[25] Ectopic expression of the bHLH gene Math1 disturbs neural development [J].

Isaka, F ;

Ishibashi, M ;

Taki, W ;

Hashimoto, N ;

Nakanishi, S ;

Kageyama, R .

EUROPEAN JOURNAL OF NEUROSCIENCE, 1999, 11 (07) :2582-2588

[26] Cortex-restricted disruption of NMDAR1 impairs neuronal patterns in the barrel cortex [J].

Iwasato, T ;

Datwani, A ;

Wolf, AM ;

Nishiyama, H ;

Taguchi, Y ;

Tonegawa, S ;

Knöpfel, T ;

Erzurumlu, RS ;

Itohara, S .

NATURE, 2000, 406 (6797) :726-731

[27] Emx1-specific expression of foreign genes using "knock-in" approach [J].

Jin, XL ;

Guo, HL ;

Mao, CJ ;

Atkins, N ;

Wang, H ;

Avasthi, PP ;

Tu, YT ;

Li, YQ .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 270 (03) :978-982

[28] Induced DNA recombination by Cre recombinase protein transduction [J].

Joshi, SK ;

Hashimoto, K ;

Koni, PA .

GENESIS, 2002, 33 (01) :48-54

[29] Developmental profile of kainate receptor subunit KA1 revealed by Cre expression in YAC transgenic mice [J].

Kask, K ;

Jerecic, J ;

Zamanillo, D ;

Wilbertz, J ;

Sprengel, R ;

Seeburg, PH .

BRAIN RESEARCH, 2000, 876 (1-2) :55-61

[30] Adeno-associated virus effectively mediates conditional gene modification in the brain [J].

Kaspar, BK ;

Vissel, B ;

Bengoechea, T ;

Crone, S ;

Randolph-Moore, L ;

Muller, R ;

Brandon, EP ;

Schaffer, D ;

Verma, IM ;

Lee, KF ;

Heinemann, SF ;

Gage, FH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (04) :2320-2325

← 1 2 3 4 5 6 7 →