Developmental profile of kainate receptor subunit KA1 revealed by Cre expression in YAC transgenic mice

被引：16

作者：

Kask, K ^{[1
]}

Jerecic, J ^{[1
]}

Zamanillo, D ^{[1
]}

Wilbertz, J ^{[1
]}

Sprengel, R ^{[1
]}

Seeburg, PH ^{[1
]}

机构：

[1] Max Planck Inst Med Res, Dept Mol Neurosci, D-69120 Heidelberg, Germany

来源：

BRAIN RESEARCH | 2000年 / 876卷 / 1-2期

关键词：

high-affinity kainate receptor subunit; YAC transgenic; Cre recombinase; lacZ reporter mice;

D O I：

10.1016/S0006-8993(00)02599-3

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

To determine the spatio-temporal expression in brain of the high-affinity kainate receptor subunit KA1, we generated transgenic mice expressing Cre recombinase from the KA1 gene on a chromosomally integrated 550 kb yeast artificial chromosome (YAC). Activity of the KA1 gene promoter during brain development was visualized by Cre immunohistochemistry, and by X-gal staining of P-galactosidase induced by Cre recombinase in double transgenic KA1-Cre/lacZ indicator mice. During early brain development, expression from the YAC-carried KA1-Cre transgene was observed in all major brain areas, predicting a function for KA1 in the developing central nervous system. In the adult brain, KA1-Cre transgene expression was restricted mainly to hippocampal CA3 pyramidal and dentate gyms granule cells, an adult expression pattern characteristic for the endogenous KA1 alleles. KA1-Cre transgenic mice may help in elucidating the role of flexed genes ablated in vivo in KA1 expressing neurons. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：55 / 61

页数：7

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