Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities

被引：16630

作者：

Schloss, Patrick D. ^{[1
,2
]}

Westcott, Sarah L. ^{[1
,2
]}

Ryabin, Thomas ^{[2
]}

Hall, Justine R. ^{[3
]}

Hartmann, Martin ^{[4
]}

Hollister, Emily B. ^{[5
]}

Lesniewski, Ryan A. ^{[6
]}

Oakley, Brian B. ^{[7
]}

Parks, Donovan H. ^{[8
]}

Robinson, Courtney J. ^{[1
]}

Sahl, Jason W. ^{[9
]}

Stres, Blaz ^{[10
]}

Thallinger, Gerhard G. ^{[11
]}

Van Horn, David J. ^{[1
]}

Weber, Carolyn F. ^{[12
]}

机构：

[1] Univ Michigan, Dept Microbiol & Immunol, Ann Arbor, MI 48109 USA

[2] Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA

[3] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA

[4] Univ British Columbia, Dept Microbiol & Immunol, Vancouver, BC V5Z 1M9, Canada

[5] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA

[6] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA

[7] Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England

[8] Dalhousie Univ, Fac Comp Sci, Halifax, NS, Canada

[9] Colorado Sch Mines, Golden, CO 80401 USA

[10] Univ Ljubljana, Dept Anim Sci, Ljubljana, Slovenia

[11] Graz Univ Technol, Inst Genom & Bioinformat, A-8010 Graz, Austria

[12] Louisiana State Univ, Dept Biol Sci, Baton Rouge, LA 70803 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2009年 / 75卷 / 23期

基金：

美国国家科学基金会;

关键词：

RIBOSOMAL-RNA; SEQUENCE DATA; DIVERSITY; ARB; LIBRARIES; DATABASE; PROGRAM; PROJECT; UNIFRAC; GENES;

D O I：

10.1128/AEM.01541-09

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

mothur aims to be a comprehensive software package that allows users to use a single piece of software to analyze community sequence data. It builds upon previous tools to provide a flexible and powerful software package for analyzing sequencing data. As a case study, we used mothur to trim, screen, and align sequences; calculate distances; assign sequences to operational taxonomic units; and describe the alpha and beta diversity of eight marine samples previously characterized by pyrosequencing of 16S rRNA gene fragments. This analysis of more than 222,000 sequences was completed in less than 2 h with a laptop computer.

引用

页码：7537 / 7541

页数：5

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