Fibroblast growth factor-2 in remodeling of the developing basement membrane zone in the trachea of infant rhesus monkeys sensitized and challenged with allergen

被引：24

作者：

Evans, MJ ^{[1
]}

Van Winkle, LS ^{[1
]}

Fanucchi, MV ^{[1
]}

Baker, GL ^{[1
]}

Murphy, AE ^{[1
]}

Nishio, SJ ^{[1
]}

Schelegle, ES ^{[1
]}

Gershwin, LJ ^{[1
]}

Sannes, PL ^{[1
]}

Plopper, CG ^{[1
]}

机构：

[1] Univ Calif Davis, Sch Vet Med, Dept Anat Physiol & Cell Biol, Ctr Comparat Resp Biol & Med, Davis, CA 95616 USA

来源：

LABORATORY INVESTIGATION | 2002年 / 82卷 / 12期

关键词：

D O I：

10.1097/01.LAB.0000043911.94235.F3

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Remodeling of the epithelial basement membrane zone (BMZ) involves increased deposition of collagen, resulting in thickening of the BMZ. The current study focuses on fibroblast growth factor-2 (FGF-2) in the tracheal BMZ in house dust mite allergen (HDMA)-sensitized infant rhesus monkeys, challenged with HDMA at a time when the BMZ is undergoing active postnatal development. To detect structural changes in the BMZ, we measured collagens I, III, and V. To detect changes in the function of the BMZ, we measured immunoreactivity of the heparan sulfate proteoglycan, perlecan, and FGF-2. We found significant thickening of the tracheal BMZ (p < 0.05) with each of these parameters. We also found that all HDMA tracheal samples expressed thin focal areas of the BMZ associated with leukocyte trafficking. These areas were depleted of perlecan and FGF-2; however, increased FGF-2 immunoreactivity was present in the adjacent basal cells. We conclude that basal cells and FGF-2 are involved with significant remodeling of the BMZ in the developing trachea of infant rhesus monkeys exposed to HDMA.

引用

页码：1747 / 1754

页数：8

共 40 条

[31] Peptidase allergens, occludin and claudins. Do their interactions facilitate the development of hypersensitivity reactions at mucosal surfaces? [J].