Modulation of nitric oxide bioavailability by erythrocytes

被引:150
作者
Huang, KT
Han, TH
Hyduke, DR
Vaughn, MW
Van Herle, H
Hein, TW
Zhang, CH
Kuo, L
Liao, JC
机构
[1] Univ Calif Los Angeles, Dept Chem Engn, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, Biomed Engn Interdepartmental Program, Los Angeles, CA 90095 USA
[3] Texas A&M Univ, Sch Med, Dept Med Physiol, College Stn, TX 77843 USA
关键词
D O I
10.1073/pnas.201276698
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Nitric oxide (NO) activates soluble guanylyl cyclase in smooth muscle cells to induce vasodilation in the vasculature. However, as hemoglobin (Hb) is an effective scavenger of NO and is present in high concentrations inside the red blood cell (RBC), the bioavailability of NO would be too low to elicit soluble guanylyl cyclase activation in the presence of blood. Therefore, NO bioactivity must be preserved. Here we present evidence suggesting that the RBC participates in the preservation of No bioactivity by reducing NO influx. The NO uptake by RBCs was increased and decreased by altering the degree of band 3 binding to the cytoskeleton. Methemoglobin and denatured hemoglobin binding to the RBC membrane or cytoskeleton also were shown to contribute to reducing the NO uptake rate of the RBC. These alterations in NO uptake by the RBC, hence the NO bioavailability, were determined to correlate with the vasodilation of isolated blood vessels. Our observations suggest that RBC membrane and cytoskeleton associated NO-inert proteins provide a barrier for NO diffusion and thus account for the reduction in the NO uptake rate of RBCs.
引用
收藏
页码:11771 / 11776
页数:6
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