Arrangement of F-actin and microtubules in the pseudopodia of Cryptochlora perforans (Chlorarachniophyta)

被引:3
作者
Dietz, C [1 ]
Schnetter, R [1 ]
机构
[1] UNIV GIESSEN, INST BOT 1, D-35390 GIESSEN, GERMANY
关键词
Chlorarachniophyta; Cryptochlora perforans; cytoskeleton; immunofluorescence; inhibitors;
D O I
10.1007/BF01276637
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The distribution of actin and the arrangement of microtubules within the filopodia of amoeboid stages of Chlorarachniophyta were studied in Cryptochlora a perforans by indirect immunofluorescence. Actin is located along the whole pseudopodium, but at different concentrations. Microtubules run like coiled cables throughout the length of the pseudopodium. At the leading edges the pseudopodium frequently appears fan-shaped and the microtubules then show a spread-our arrangement, but they do not reach the cytoplasm front. Colchicine inhibited particle motility in the filopodia. The particle transport seems to be insensitive to cytochalasin D, but cells contracted their filopodia.
引用
收藏
页码:82 / 90
页数:9
相关论文
共 38 条
[31]   GROWTH OF NEURITES WITHOUT FILOPODIAL OR LAMELLIPODIAL ACTIVITY IN THE PRESENCE OF CYTOCHALASIN-B [J].
MARSH, L ;
LETOURNEAU, PC .
JOURNAL OF CELL BIOLOGY, 1984, 99 (06) :2041-2047
[32]   TURNOVER OF FLUORESCENTLY LABELED TUBULIN AND ACTIN IN THE AXON [J].
OKABE, S ;
HIROKAWA, N .
NATURE, 1990, 343 (6257) :479-482
[33]   LOCATION OF ACTIN, MYOSIN, AND MICROTUBULAR STRUCTURES DURING DIRECTED LOCOMOTION OF DICTYOSTELIUM AMEBAE [J].
RUBINO, S ;
FIGHETTI, M ;
UNGER, E ;
CAPPUCCINELLI, P .
JOURNAL OF CELL BIOLOGY, 1984, 98 (02) :382-390
[34]   NATURALLY-OCCURRING TUBULIN-CONTAINING PARACRYSTALS IN ALLOGROMIA - IMMUNOCYTOCHEMICAL IDENTIFICATION AND FUNCTIONAL-SIGNIFICANCE [J].
RUPP, G ;
BOWSER, SS ;
MANNELLA, CA ;
RIEDER, CL .
CELL MOTILITY AND THE CYTOSKELETON, 1986, 6 (04) :363-375
[35]  
Stadelmann E.J., 1972, METHODS CELL PHYSL, V5, P325, DOI DOI 10.1016/S0091-679X(08)60717-7
[36]   A NEW MODEL OF RETICULOPODIAL MOTILITY AND SHAPE - EVIDENCE FOR A MICROTUBULE-BASED MOTOR AND AN ACTIN SKELETON [J].
TRAVIS, JL ;
BOWSER, SS .
CELL MOTILITY AND THE CYTOSKELETON, 1986, 6 (01) :2-14
[37]   MICROTUBULE-DEPENDENT RETICULOPODIAL MOTILITY - IS THERE A ROLE FOR ACTIN [J].
TRAVIS, JL ;
BOWSER, SS .
CELL MOTILITY AND THE CYTOSKELETON, 1986, 6 (02) :146-152
[38]   LOCALIZATION OF ACTIN AND MYOSIN FOR THE STUDY OF AMEBOID MOVEMENT IN DICTYOSTELIUM USING IMPROVED IMMUNOFLUORESCENCE [J].
YUMURA, S ;
MORI, H ;
FUKUI, Y .
JOURNAL OF CELL BIOLOGY, 1984, 99 (03) :894-899