Immobilization of horseradish peroxidase via an amino silane on oxidized ultrananocrystalline diamond

被引：50

作者：

Hernando, Jorge

Pourrostami, Tahmineh

Garrido, Jose A.

Williams, Oliver A.

Gruen, Dieter M.

Kromka, Alexander

Steinmueller, Doris

Stutzmann, Martin

机构：

[1] Tech Univ Munich, Walter Schottky Inst, D-85748 Garching, Germany

[2] Argonne Natl Lab, Ctr Nanoscale Mat, Argonne, IL 60439 USA

[3] Argonne Natl Lab, Mat Sci & Chem Div, Argonne, IL 60439 USA

[4] Hartstoffbeschichtungs GmbH, rBest Coating, A-6150 Steinach, Austria

来源：

关键词：

diamond film; nanocrystalline; electrochemical; biomaterials;

D O I：

10.1016/j.diamond.2006.04.005

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

We discuss the complete functionalization of nitrogen-doped ultrananocrystalline diamond (UNCD) films, starting from an oxidized surface. First, the presence of hydroxyl groups on oxidized nanocrystalline diamond (NCD) was confirmed by fluorescence microscopy. Next, the grafting of a linker molecule such as 3-aminopropylmethyldiethoxysilane on oxidized NCD was confirmed by fluorescence microscopy and X-ray photoelectron spectroscopy (XPS). Then the horseradish peroxidase (HRP) enzyme was immobilized on silane-modified initially oxidized UNCD. The HRP-modified UNCD was characterized by electrochemical techniques, such as faradaic cyclic voltammetry and the amperometric response to H2O2. This response to H2O2 is discussed in terms of the layer-by-layer configuration used and the electronic properties of conducting UNCD. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：138 / 143

页数：6

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[1] Capacitance measurements of antibody-antigen interactions in a flow system [J].