Identification and characterization of a novel cell cycle-regulated internal ribosome entry site

被引：290

作者：

Cornelis, S

Bruynooghe, Y

Denecker, G

Van Huffel, S

Tinton, S

Beyaert, R

机构：

[1] Flanders Interuniv Inst, Dept Biol Mol, B-9000 Ghent, Belgium

[2] State Univ Ghent, B-9000 Ghent, Belgium

来源：

MOLECULAR CELL | 2000年 / 5卷 / 04期

关键词：

D O I：

10.1016/S1097-2765(00)80239-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

PITSLRE protein kinases are related to the large family of cyclin-dependent kinases. They have been proposed to act as tumor suppressor genes and have been shown to play a role in cell cycle progression. We report that two PITSLRE protein kinase isoforms, namely p110(PITSLRE) and p58(PITSLRE), are translated from a single transcript by initiation at alternative in-frame AUG codons. p110(PITSLRE) is produced by classical cap-dependent translation, whereas p58(PITSLRE) results from internal initiation of translation controlled by an internal ribosome entry site (IRES) with unique properties. The IRES element is localized to the mRNA coding region, and its activity is cell cycle regulated, which permits translation of p58(PITSLRE) in G2/M.

引用

页码：597 / 605

页数：9

共 44 条

[1]

Regulation of vascular endothelial growth factor (VEGF) expression is mediated by internal initiation of translation and alternative initiation of transcription [J].