Skin unhairing proteases of Bacillus subtilis: production and partial characterization

被引：47

作者：

Varela, H

Ferrari, MD

Belobrajdic, L

Vazquez, A

Loperena, ML

机构：

[1] Depto. Bioingeniería, Inst. de Ing. Química, Universidad de la República, 11300, Montevideo

来源：

BIOTECHNOLOGY LETTERS | 1997年 / 19卷 / 08期

关键词：

D O I：

10.1023/A:1018384025181

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Proteases able to unhair sheep skins were produced from Bacillus subtilis. Protease activity was increased from 640 to 990 U/mL by using a fed-batch culture with glucose added sequentially up to 10 g/L. The crude enzyme preparation was mainly a mixture of metallo- and serine-proteases with optimal pH and temperature for protease activity at 7 and 50-55 degrees C, respectively. At pH 8 and 30 degrees C, protease activity of the crude enzyme was 33% of the maximal value and 97% of the original activity (900 U/mL) was retained after incubation for 4 hours.

引用

页码：755 / 758

页数：4

共 7 条

[1]

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