Parallel confocal detection of single molecules in real time

被引：70

作者：

Lundquist, Paul M. ^{[1
]}

Zhong, Cheng F. ^{[1
]}

Zhao, Peiqian ^{[1
]}

Tomaney, Austin B. ^{[1
]}

Peluso, Paul S. ^{[1
]}

Dixon, John ^{[1
]}

Bettman, Brad ^{[1
]}

Lacroix, Yves ^{[1
]}

Kwo, Deborah P. ^{[1
]}

McCullough, Etienne ^{[1
]}

Maxham, Mark ^{[1
]}

Hester, Kevin ^{[1
]}

McNitt, Paul ^{[1
]}

Grey, Donald M. ^{[1
]}

Henriquez, Carlos ^{[1
]}

Foquet, Mathieu ^{[1
]}

Turner, Stephen W. ^{[1
]}

Zaccarin, Denis ^{[1
]}

机构：

[1] Pacific Biosci Inc, Menlo Pk, CA 94025 USA

来源：

OPTICS LETTERS | 2008年 / 33卷 / 09期

关键词：

D O I：

10.1364/OL.33.001026

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

The confocal detection principle is extended to a highly parallel optical system that continuously analyzes thousands of concurrent sample locations. This is achieved through the use of a holographic laser illumination multiplexer combined with a confocal pinhole array before a prism dispersive element used to provide spectroscopic information from each confocal volume. The system is demonstrated to detect and identify single fluorescent molecules from each of several thousand independent confocal volumes in real time. (C) 2008 Optical Society of America.

引用

页码：1026 / 1028

页数：3

共 14 条

[1] Dependence of transverse and longitudinal resolutions on incident Gaussian beam widths in the illumination part of optical scanning microscopy [J].