Chronic effects of berberine on blood, liver glucolipid metabolism and liver PPARs expression in diabetic hyperlipidemic rats

被引：133

作者：

Zhou, Ji Yin ^{[1
]}

Zhou, Shi Wen ^{[1
]}

Bin Zhang, Ke ^{[1
]}

Tang, Jian Lin ^{[1
]}

Guang, Li Xia ^{[1
]}

Ying, Yi ^{[1
]}

Xu, Ying ^{[1
]}

Zhang, Le ^{[1
]}

Li, Dan Dan ^{[1
]}

机构：

[1] Third Mil Med Univ, Xinqiao Hosp, Chongqing 400037, Peoples R China

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2008年 / 31卷 / 06期

关键词：

berberine; liver; peroxisome proliferator-activated receptor; type 2 diabetes mellitus; hyperglycermia; hyperlipidemia;

D O I：

10.1248/bpb.31.1169

中图分类号：

R9 [药学];

学科分类号：

1007 [药学];

摘要：

Berberine is one of the main alkaloids of Rhizoma coptidis which has been used as a folk medicine to treat diabetes mellitus for more than 1400 years in China. To investigate the chronic effect of berberine on diabetic hyperlipidemic rats, fasted rats were intraperitoneally injected 35 mg/kg streptozotocin. Diabetic rats were admitted after 2 weeks and given a high-carbohydrate/high-fat diet to induce hyperlipidemia. The rats were divided into 7 groups at the end of week 16: normal and diabetic rats received no drug, 5 treatment groups were administered with either 75, 150, 300 mg/kg berberine, 100 mg/kg fenofibrate or 4 mg/kg rosiglitazone per day for 16 weeks, respectively. The blood glucose, hemoglobin Ale, lipid metabolic parameters and hepatic glycogen and triglyceride were measured, and histopathology and peroxisome proliferator-activated receptors (PPARs) alpha/delta/gamma expression of liver were determined by hematoxylin eosin and immunohistochemical staining. Berberine reduced diabetic rats' body weight, liver weight and liver to body weight ratio. Berberine restored the increased blood glucose, hemoglobin A1c, total cholesterol, triglyceride, low density lipoprotein-cholesterol, apolipoprotein B and the decreased high density lipoprotein-cholesterol, apolipoprotein At levels in diabetic rats to near the control ones. Berberine alleviated the pathological progression of liver and reverted the increased hepatic glycogen and triglyceride to near the control levels. Berberine increased PPAR alpha/delta expression and reduced PPAR gamma expression in liver of diabetic rat to near the control ones. Berberine improved glucolipid metabolism both in blood and liver in diabetic rats possibly through modulating the metabolic related PPAR alpha/delta/gamma protein expression in liver.

引用

页码：1169 / 1176

页数：8

共 37 条

[1]

Activation of peroxisome proliferator-activated receptor α increases the expression and activity of microsomal triglyceride transfer protein in the liver [J].

Améen, C ;

Edvardsson, U ;

Ljungberg, A ;

Asp, L ;

Åkerblad, P ;

Tuneld, A ;

Olofsson, SO ;

Lindén, D ;

Oscarsson, J .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (02) :1224-1229

[2]

[Anonymous], 2000, ALTERN MED REV, V5, P175

[3]

Arvind K, 2002, INDIAN J MED RES, V116, P163

[4]

The mechanisms of action of PPARs [J].

Berger, J ;

Moller, DE .

ANNUAL REVIEW OF MEDICINE, 2002, 53 :409-435

[5]

Inhibition of lipid synthesis through activation of AMP kinase: an additional mechanism for the hypolipidemic effects of berberine [J].

Brusq, Jean-Marie ;

Ancellin, Nicolas ;

Grondin, Pascal ;

Guillard, Raphaelle ;

Martin, Sandrine ;

Saintillan, Yannick ;

Issandou, Marc .

JOURNAL OF LIPID RESEARCH, 2006, 47 (06) :1281-1288

[6]

Damci Taner, 2003, Eur J Intern Med, V14, P357, DOI 10.1016/S0953-6205(03)90001-X

[7]

DEPAEPE ME, 1995, HEPATOLOGY, V21, P1144, DOI 10.1002/hep.1840210438

[8]

Pioglitazone prevents hypertension and reduces oxidative stress in diet-induced obesity [J].

Dobrian, AD ;

Schriver, SD ;

Khraibi, AA ;

Prewitt, RL .

HYPERTENSION, 2004, 43 (01) :48-56

[9]

ELISAF MS, 1995, DIALYSIS TRANSPLANT, V24, P642

[10]

PPAR-α effects on the heart and other vascular tissues [J].

Francis, GA ;

Annicotte, JS ;

Auwerx, J .

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2003, 285 (01) :H1-H9

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