Enantiomeric separation of underivatized small amines in conventional and on-chip capillary electrophoresis with contactless conductivity detection

被引：47

作者：

Gong, Xiao Yang ^{[1
]}

Hauser, Peter C. ^{[1
]}

机构：

[1] Univ Basel, Dept Chem, CH-4004 Basel, Switzerland

来源：

ELECTROPHORESIS | 2006年 / 27卷 / 21期

关键词：

amines; capillary electrophoresis; contactless conductivity detection; enantiomeric separation;

D O I：

10.1002/elps.200600258

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The determination of the enantiomers of small non-UV-absorbing amines which otherwise can only be achieved with difficulty was possible by using a combination of the chiral crown ether (+)-(18-crown-6)-2,3,11,12-tetracarboxylic acid (18C(6)H(4)) and dimethyl-beta-CD as selectors in CE and contactless conductivity measurement for detection. Alkylamines without any other functional group, amino alcohols, species with ether or ester groups and with a cyclic moiety were investigated. The detection limits were found to be about 1.0 mu M and the determination is possible up to at least 1.0 mM. The determination of enantiomeric ratios of up to 99.5:0.5 was also found feasible.

引用

页码：4375 / 4382

页数：8

共 40 条

[1] Mechanism of capillary electrophoresis enantioseparations using a combination of an achiral crown ether plus cyclodextrins [J].