Induction of jasmonate biosynthesis in arbuscular mycorrhizal barley roots

被引:150
作者
Hause, B [1 ]
Maier, W
Miersch, O
Kramell, R
Strack, D
机构
[1] Leibniz Inst Pflanzenbiochem, Abt Sekundarstoffwechsel, D-06120 Halle An Der Saale, Germany
[2] Leibniz Inst Pflanzenbiochem, Abt Naturstoff Biotechnol, D-06120 Halle An Der Saale, Germany
关键词
D O I
10.1104/pp.006007
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Colonization of barley (Hordeum vulgare cv Salome) roots by an arbuscular mycorrhizal fungus, Glomus intraradices Schenck & Smith, leads to elevated levels of endogenous jasmonic acid (JA) and its amino acid conjugate JA-isoleucine, whereas the level of the JA precursor, oxophytodienoic acid, remains constant. The rise in jasmonates is accompanied by the expression of genes coding for an enzyme of JA biosynthesis (allene oxide synthase) and of a jasmonate-induced protein (JIP23). In situ hybridization and immunocytochemical analysis revealed that expression of these genes occurred cell specifically within arbuscule-containing root cortex cells. The concomitant gene expression indicates that jasmonates are generated and act within arbuscule-containing cells. By use of a near-synchronous mycorrhization, analysis of temporal expression patterns showed the occurrence of transcript accumulation 4 to 6 d after the appearance of the first arbuscules. This suggests that the endogenous rise in jasmonates might be related to the fully established symbiosis rather than to the recognition of interacting partners or to the onset of interaction. Because the plant supplies the fungus with carbohydrates, a model is proposed in which the induction of JA biosynthesis in colonized roots is linked to the stronger sink function of mycorrhizal roots compared with nonmycorrhizal roots.
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页码:1213 / 1220
页数:8
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