Circular dichroism spectra demonstrate formation of the thrombin-binding DNA aptamer G-quadruplex under stabilizing-cation-deficient conditions

被引：145

作者：

Nagatoishi, Satoru ^{[1
]}

Tanaka, Yoshikazu ^{[1
]}

Tsumoto, Kouhei ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Frontier Sci, Dept Med Genome Sci, Kashiwa, Chiba 2778562, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 352卷 / 03期

关键词：

circular dichroism; G-quadruplex; solvent; DNA; aptamer;

D O I：

10.1016/j.bbrc.2006.11.088

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It is noteworthy that the formation of the DNA G-quadruplex is induced by factors other than stabilizing cations because this event probably occurs in living cells. Previous studies have shown that thrombin-binding DNA aptamer (TBA) forms a chair-type intramolecular G-quadruplex structure that binds with thrombin protein in the absence of stabilizing cations. Here, we used circular dichroism (CD) spectroscopy to confirm G-quadruplex formation in the presence of thrombin without stabilizing cations. We obtained characteristic CD spectra that demonstrated that TBA forms the distinctive G-quadruplex structure. Additionally, we investigated G-quadruplex formation induced by change of solvent environment: the influence of low-temperature conditions and molecular crowding. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：812 / 817

页数：6

共 45 条

[1] Ability of thrombin to act as molecular chaperone, inducing formation of quadruplex structure of thrombin-binding aptamer [J].

Baldrich, E ;

O'Sullivan, CK .

ANALYTICAL BIOCHEMISTRY, 2005, 341 (01) :194-197

[2] Aptasensor development: Elucidation of critical parameters for optimal aptamer performance [J].

Baldrich, E ;

Restrepo, A ;

O'Sullivan, CK .

ANALYTICAL CHEMISTRY, 2004, 76 (23) :7053-7063

[3] Activity of water in aqueous systems; A frequently neglected property [J].

Blandamer, MJ ;

Engberts, JBFN ;

Gleeson, PT ;

Reis, JCR .

CHEMICAL SOCIETY REVIEWS, 2005, 34 (05) :440-458

[4] SELECTION OF SINGLE-STRANDED-DNA MOLECULES THAT BIND AND INHIBIT HUMAN THROMBIN [J].

BOCK, LC ;

GRIFFIN, LC ;

LATHAM, JA ;

VERMAAS, EH ;

TOOLE, JJ .

NATURE, 1992, 355 (6360) :564-566

[5] THE BETA-SUBUNIT OF OXYTRICHA TELOMERE-BINDING PROTEIN PROMOTES G-QUARTET FORMATION BY TELOMERIC DNA [J].

FANG, GW ;

CECH, TR .

CELL, 1993, 74 (05) :875-885

[6] PROMOTION OF PARALLEL DNA QUADRUPLEXES BY A YEAST TELOMERE BINDING-PROTEIN - A CIRCULAR-DICHROISM STUDY [J].

GIRALDO, R ;

SUZUKI, M ;

CHAPMAN, L ;

RHODES, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (16) :7658-7662

[7] Metabolic buffering exerted by macromolecular crowding on DNA-DNA interactions: Origin and physiological significance [J].

Goobes, R ;

Kahana, N ;

Cohen, O ;

Minsky, A .

BIOCHEMISTRY, 2003, 42 (08) :2431-2440

[8] Aptamer beacons for the direct detection of proteins [J].

Hamaguchi, N ;

Ellington, A ;

Stanton, M .

ANALYTICAL BIOCHEMISTRY, 2001, 294 (02) :126-131

[9] Optical sensors based on hybrid aptamer/conjugated polymer complexes [J].

Ho, HA ;

Leclerc, M .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (05) :1384-1387

[10] DNA G-quartets in a 1.86 Å resolution structure of an Oxytricha nova telomeric protein-DNA complex [J].

Horvath, MP ;

Schultz, SC .

JOURNAL OF MOLECULAR BIOLOGY, 2001, 310 (02) :367-377

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