Antioxidant and cytoprotective effect of peptides produced by hydrolysis of whey protein concentrate with trypsin

被引：81

作者：

Ballatore, Maria Belen ^{[1
,5
]}

Bettiol, Marina del Rosario ^{[2
,5
]}

Vanden Braber, Noelia L. ^{[2
,5
]}

Aminahuel, Carla Aylen ^{[2
,5
]}

Rossi, Yanina Estefania ^{[2
,5
]}

Petroselli, Gabriela ^{[3
,4
]}

Erra-Balsells, Rosa ^{[3
,4
]}

Cavaglieri, Lilia Rene ^{[1
,5
]}

Montenegro, Mariana Angelica ^{[2
,5
]}

机构：

[1] Univ Nacl Rio Cuarto, Dept Microbiol & Inmunol, Cordoba, Argentina

[2] Univ Nacl Villa Maria, CITVM, CONICET, Cordoba, Argentina

[3] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Quim Organ, Pabellon 2,3er P,Ciudad Univ, RA-1428 Buenos Aires, DF, Argentina

[4] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Ctr Invest Hidratos Carbono CIHIDECAR, CONICET, Pabellon 2,3er P,Ciudad Univ, RA-1428 Buenos Aires, DF, Argentina

[5] Consejo Nacl Invest Cient & Tecn, CIC, Buenos Aires, DF, Argentina

来源：

FOOD CHEMISTRY | 2020年 / 319卷

关键词：

Whey protein; Hydrolysis; Trypsin; Peptides; Antioxidants; Cytoprotection; BIOACTIVE PEPTIDES; OXIDATIVE STRESS; ASSAY;

D O I：

10.1016/j.foodchem.2020.126472

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Whey protein is one of the most relevant co-products manufactured by the dairy industry and it is a powerful environmental pollutant. Therefore, the enzymatic hydrolysis of whey protein concentrate (WPC 35) to produce antioxidant peptides is an innovative approach which can provide added value to whey. The WPC 35 hydrolysis with trypsin was carried out for 4.31 hat 41.1 degrees C with an enzyme/substrate ratio of 0.017. Under such hydrolysis conditions, the peptides produced have the highest radical scavenging activity and cytoprotector effect. The WPC hydrolysate and a permeate <= 3 kDa were characterized by SDS-page, RP-HPLC and MALDI-TOF-MS. Furthermore, O-2(center dot-) and HO. scavenging activity and the cytoprotective effect against a stress agent in epithelial cells of the rat ileum (IEC-18) were determined. In this study, strong antioxidant and cytoprotective peptides were obtained from a low-cost dairy industry product, which could improve consumers' health when used as functional ingredients.

引用

页数：11

共 41 条

[31]

Antioxidant activity of peptide fractions from whey protein hydrolysates as measured by electron spin resonance [J].