Genome-Wide Survey and Expression Analysis of the Plant-Specific NAC Transcription Factor Family in Soybean During Development and Dehydration Stress

被引:345
作者
Dung Tien Le [1 ,2 ]
Nishiyama, Rie [1 ]
Watanabe, Yasuko [1 ]
Mochida, Keiichi [3 ]
Yamaguchi-Shinozaki, Kazuko [4 ]
Shinozaki, Kazuo [3 ]
Lam-Son Phan Tran [1 ]
机构
[1] RIKEN Yokohama Inst, Plant Sci Ctr, Signaling Pathway Res Unit, Yokohama, Kanagawa 2300045, Japan
[2] Vietnamese Acad Agr Sci, Agr Genet Inst, Hanoi, Vietnam
[3] RIKEN Yokohama Inst, Plant Sci Ctr, Gene Discovery Res Grp, Yokohama, Kanagawa 2300045, Japan
[4] Japan Int Ctr Agr Sci, Ibaraki 3058686, Japan
关键词
soybean; NAC transcription factors; dehydration; sequence analysis; expression analysis; GLYCINE-MAX; REGULATORY NETWORKS; ARABIDOPSIS-THALIANA; FUNCTIONAL-ANALYSIS; DROUGHT RESISTANCE; GENES; SEQUENCE; IDENTIFICATION; PREDICTION; TOLERANCE;
D O I
10.1093/dnares/dsr015
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Plant-specific NAC transcription factors (TFs) play important roles in regulating diverse biological processes, including development, senescence, growth, cell division and responses to environmental stress stimuli. Within the soybean genome, we identified 152 full-length GmNAC TFs, including 11 membrane-bound members. In silico analysis of the GmNACs, together with their Arabidopsis and rice counterparts, revealed similar NAC architecture. Next, we explored the soybean Affymetrix array and Illumina transcriptome sequence data to analyse tissue-specific expression profiles of GmNAC genes. Phylogenetic analysis using stress-related NAC TFs from Arabidopsis and rice as seeding sequences identified 58 of the 152 GmNACs as putative stress-responsive genes, including eight previously reported dehydration-responsive GmNACs. We could design gene-specific primers for quantitative real-time PCR verification of 38 out of 50 newly predicted stress-related genes. Twenty-five and six GmNACs were found to be induced and repressed 2-fold or more, respectively, in soybean roots and/or shoots in response to dehydration. GmNAC085, whose amino acid sequence was 39%; identical to that of well-known SNAC1/ONAC2, was the most induced gene upon dehydration, showing 390-fold and 20-fold induction in shoots and roots, respectively. Our systematic analysis has identified excellent tissue-specific and/or dehydration-responsive candidate GmNAC genes for in-depth characterization and future development of improved drought-tolerant transgenic soybeans.
引用
收藏
页码:263 / 276
页数:14
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