Evidence for the localization of the Arabidopsis cytokinin receptors AHK3 and AHK4 in the endoplasmic reticulum

被引:134
作者
Caesar, Katharina [1 ]
Thamm, Antje M. K. [1 ]
Witthoeft, Janika [1 ]
Elgass, Kirstin [1 ]
Huppenberger, Peter [1 ]
Grefen, Christopher [2 ]
Horak, Jakub [3 ]
Harter, Klaus [1 ]
机构
[1] Univ Tubingen, Dept Plant Physiol, Ctr Plant Mol Biol, D-72076 Tubingen, Germany
[2] Univ Glasgow, Inst Mol Cell & Syst Biol, Glasgow G12 8QQ, Lanark, Scotland
[3] Masaryk Univ, Fac Sci, Brno 62500, Czech Republic
关键词
AHK3; cytokinin perception; endoplasmic reticulum; FIDSAM; EQUILIBRATIVE NUCLEOSIDE TRANSPORTER; PROTEIN-PROTEIN INTERACTIONS; AUXIN-BINDING PROTEIN-1; HISTIDINE KINASE; SUBCELLULAR-LOCALIZATION; FLUORESCENCE MICROSCOPY; FUNCTIONAL-ANALYSIS; PLASMA-MEMBRANE; PATHWAY; STRESS;
D O I
10.1093/jxb/err238
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Cytokinins are hormones that are involved in various processes of plant growth and development. The model of cytokinin signalling starts with hormone perception through membrane-localized histidine kinase receptors. Although the biochemical properties and functions of these receptors have been extensively studied, there is no solid proof of their subcellular localization. Here, cell biological and biochemical evidence for the localization of functional fluorophor-tagged fusions of Arabidopsis histidine kinase 3 (AHK3) and 4 (AHK4), members of the cytokinin receptor family, in the endoplasmic reticulum (ER) is provided. Furthermore, membrane-bound AHK3 interacts with AHK4 in vivo. The ER localization and putative function of cytokinin receptors from the ER have major impacts on the concept of cytokinin perception and signalling, and hormonal cross-talk in plants.
引用
收藏
页码:5571 / 5580
页数:10
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