Feasibility of using carboxylic-rich polymeric surfaces for the covalent binding of oligonucleotides for microPCR applications

被引:20
作者
Ivanova, EP
Papiernik, M
Oliveira, A
Sbarski, I
Smekal, T
Grodzinski, P
Nicolau, DV
机构
[1] Swinburne Univ Technol, Hawthorn, Vic 3122, Australia
[2] Motorola Phys Sci Res Labs, Microfluid Lab, Phoenix, AZ USA
关键词
D O I
10.1088/0964-1726/11/5/325
中图分类号
TH7 [仪器、仪表];
学科分类号
0804 ; 080401 ; 081102 ;
摘要
The chemical binding of oligonucleotide/DNA on poly styrene-related polymeric surfaces has been investigated using contact angle measurements, x-ray photoelectron spectroscopy and gravimetric analysis. The results of the covalent attachment of the phosphorylated oligonucleotides using the hetero-bifunctional cross linker 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride to polystyrene-co-maleic anhydride (PSMAA) and polystyrene-co-maleic acid (PSMA) are described. The immobilization efficiency of covalently coupled 26-mer oligonucleotides to polymeric surfaces was estimated as 0.3 x 10(10) and 0.1 x 10(9) molecules mm(-2) for PSMA and PSMAA, respectively. The results suggest that, although the covalent binding on PSMAA per se is not capable of the high density of DNA required by micro-PCR applications, the method has the potential to be used as a cheap alternative for other low-cost, less DNA-sensitive applications such as disposable biosensors.
引用
收藏
页码:783 / 791
页数:9
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