Rapid and selective determination of ammonium by fluorimetric flow injection analysis

被引:30
作者
Mana, H [1 ]
Spohn, U [1 ]
机构
[1] Univ Halle, Inst Biotechnol, D-06120 Halle, Germany
来源
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 2000年 / 366卷 / 08期
关键词
D O I
10.1007/s002160051579
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Selective and sensitive procedures for the determination of ammonium in river water and diluted urine were developed by using flow injection analysis equipment. The methods are based on the derivatization of ammonia with o-phthaldehyde (OPA) and thioglycolate under alkaline conditions. The formed isoindole derivative is detected fluorimetrically at an excitation wavelength of 415 nm and an emission wavelength of 485 nm. The derivatization only takes 15 to 20 s at room temperature to achieve the maximum sensitivity. The optimized OPA reagent shows a surprisingly high selectivity for ammonium in the presence of many primary amines. With respect to the analysis of turbid and fluorescent sample solutions the selectivity can be improved by separating the ammonia through a microporous membrane from the OPA reagent. Without this separation step ammonia can be detected in the range between 0.05 and 100 mu M with excellent linearity. After the insertion of an optimized membrane separation cell ammonia can be determined in the linear range between 0.2 mu M and 20 mM.
引用
收藏
页码:825 / 829
页数:5
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